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首页> 外文期刊>Veterinary Dermatology >Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions.
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Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions.

机译：犬真皮乳头细胞的分子生物学和免疫组织学表征以及培养条件的评估。

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The dermal papilla (DP) plays pivotal roles in hair follicle morphogenesis and cycling. However, our understanding of the biology of the canine DP is extremely limited. The aim of this study was to elucidate molecular biological and immunohistochemical characteristics of canine DP cells and determine appropriate conditions for in vitro expansion. Histological investigation revealed that the canine DP expressed biomarkers of human and rodent DP, including alkaline phosphatase (ALP) and versican. When microdissected, canine DP, but not fibroblasts, strongly expressed the DP-related genes for alkaline phosphatase, Wnt inhibitory factor 1 and lymphoid enhancer-binding factor 1, confirming successful isolation. The growth rate of isolated canine DP cells was moderate in conventional culture conditions for rodent and human DP; however, AmnioMAX-C100 complete medium allowed more efficient cultivation. Dermal papilla marker gene expression was maintained in early passage cultured DP cells, but gradually lost after the third passage. Approaches to mimic the in vivo DP environment in culture, such as supplementation of keratinocyte-conditioned medium or use of extracellular matrix-coated dishes, moderately ameliorated loss of DP gene expression in canine DP cells. It is possible that constituent factors in AmnioMAX may influence culture. These findings suggested that further refinements of culture conditions may enable DP cell expansion without impairing intrinsic properties and, importantly, demonstrated that AmnioMAX-cultured early passage canine DP cells partly maintained the biological characteristics of in vivo canine DP cells. This study provides crucial information necessary for further optimization of culture conditions of canine DP.
机译：真皮乳头（DP）在毛囊形态发生和循环中起关键作用。但是，我们对犬DP生物学的了解极为有限。这项研究的目的是阐明犬DP细胞的分子生物学和免疫组化特征，并确定体外扩增的适当条件。组织学研究表明，犬DP表达了人类和啮齿动物DP的生物标记，包括碱性磷酸酶（ALP）和versican。当进行显微解剖时，犬DP而不是成纤维细胞强烈表达了与DP相关的碱性磷酸酶，Wnt抑制因子1和淋巴增强结合因子1的基因，证实了成功的分离。在啮齿动物和人DP的常规培养条件下，分离的犬DP细胞的生长速度中等。但是，AmnioMAX-C100完全培养基可以提高培养效率。在早期传代培养的DP细胞中维持皮肤乳头标记基因表达，但在第三次传代后逐渐丧失。模仿培养中DP体内环境的方法，例如补充角质形成细胞条件培养基或使用细胞外基质包被的培养皿，可适度改善犬DP细胞中DP基因表达的丧失。 AmnioMAX中的构成因素可能会影响文化。这些发现表明，进一步完善培养条件可以使DP细胞扩增而不会损害其固有特性，并且重要的是，表明AmnioMAX培养的早期传代犬DP细胞部分保留了体内犬DP细胞的生物学特性。 。这项研究提供了进一步优化犬DP培养条件所必需的重要信息。

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