• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Dermatology >Malassezia dermatitis in dogs in Brazil: diagnosis, evaluation of clinical signs and molecular identification.
【24h】

Malassezia dermatitis in dogs in Brazil: diagnosis, evaluation of clinical signs and molecular identification.

机译：巴西犬 Malassezia 皮炎：诊断，临床体征评估和分子鉴定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Skin carriage and quantification of Malassezia yeasts were evaluated in 180 healthy dogs (group 1) and 117 dogs with clinical signs (pruritus, erythema, lichenification/seborrhoea, excoriations and alopecia) that could be related to Malassezia dermatitis (group 2) in Brazil. The lesions in the group 2 dogs were evaluated using CADESI-03 scores. Samples were collected from five different anatomical areas. Direct examination was performed using the tape strip technique, and results were expressed as the mean number of yeasts per x1000 microscopic field per dog. For mycological culture, a single piece of sterilized carpet was applied to the same areas sampled for cytology, and transferred onto Dixon's modified medium. Yeast populations were expressed as mean colony forming units (CFU)/plate. Malassezia isolates were characterized by polymerase chain reaction-restriction endonuclease analysis of the large subunit (LSU) of ribosomal RNA gene. The probability of culturing Malassezia from dogs with skin lesions was significantly higher (P<0.001) than from healthy dogs. There was a linear trend between CADESI-03 score and mean CFU/plate. Group 2 dogs with positive cultures had higher CADESI-03 scores than those with negative cultures (P<0.05). Almost all isolates were identified as Malassezia pachydermatis. Only one isolate (group 2) was identified as Malassezia furfur. These data suggest that dogs with skin disorders harbouring Malassezia yeasts in quantities higher than 120 mean CFU/plate should be considered as having Malassezia dermatitis. The presence of Malassezia appears to exacerbate clinical lesions in dogs.
机译：评估了180只健康犬（第1组）和117只有可能与马拉色菌性皮炎（第2组）。使用CADESI-03评分评估第2组狗的损伤。从五个不同的解剖区域收集样品。使用胶带剥离技术进行直接检查，结果表示为每只狗每x1000显微镜视野的酵母平均数。对于真菌培养，将一块无菌地毯铺在用于细胞学检查的相同区域，然后转移到Dixon改良培养基上。酵母菌群体表示为平均菌落形成单位（CFU）/板。通过对核糖体RNA基因大亚基（LSU）的聚合酶链反应-限制性内切核酸酶分析，对 Malassezia 菌株进行了鉴定。患有皮肤病变的犬患马拉色菌的可能性显着高于健康犬（ P <0.001）。 CADESI-03得分与平均CFU /板之间存在线性趋势。具有阳性培养物的第2组犬比具有阴性培养物的犬具有更高的CADESI-03得分（ P <0.05）。几乎所有的分离物都被鉴定为帕拉氏疟原虫”。仅一个分离株（第2组）被鉴定为糠fur马拉色菌。这些数据表明，患有皮肤病的犬中含有 Malassezia 酵母的数量高于120个平均CFU /板，应将其视为患有 Malassezia皮肤炎。马拉色菌病的出现似乎加剧了犬的临床损害。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号