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首页> 外文期刊>Veterinary Microbiology >Identification of beta-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods.
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Identification of beta-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods.

机译：用蛋白质组学方法鉴定人和牛分离的金黄色葡萄球菌菌株中的β-内酰胺酶，这些菌株对耐青霉素酶的青霉素（PRPs）具有临界耐药性。

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摘要

Methicillin and oxacillin-hydrolyzing enzymes of 6 borderline methicillin-resistant and 1 methicillin-resistant Staphylococcus aureus strains isolated from human clinical samples and 4 borderline methicillin-resistant S. aureus strains isolated from bovine mastitis were investigated. As previous studies suggested the involvement of an additional enzyme besides the penicillinase BlaZ in the determination of borderline resistance, we analyzed the expressed extracellular and membrane-bound beta-lactamases with 2-D gel electrophoresis and mass spectrometry. Our analysis showed that the penicillin-hydrolyzing BlaZ alone was responsible for the hydrolysis of both methicillin and oxacillin. All supernatant and membrane fractions contained the same enzyme with slight sequence variations. The size and pI of the proteins were also variable, probably due to spontaneous hydrolysis and/or posttranslational modifications. Interestingly, we found also cytotoxins and other virulence factors in some nitrocefin-hydrolyzing dots, suggesting that those proteins might have a role in the reduction of local antibiotic concentration.
机译：从人类临床样本中分离出的6株耐甲氧西林和1株耐甲氧西林金黄色葡萄球菌和4株耐甲氧西林的耐甲氧西林和草酸水解酶。研究了从牛乳腺炎中分离出的金黄色葡萄球菌。由于先前的研究表明除青霉素酶BlaZ之外还存在其他酶来确定界线抗性，因此我们用2-D凝胶电泳和质谱分析了表达的细胞外和膜结合的β-内酰胺酶。我们的分析表明，单独水解青霉素的BlaZ负责甲氧西林和奥沙西林的水解。所有上清液和膜级分均含有相同的酶，但序列略有差异。蛋白质的大小和pI也是可变的，可能是由于自发水解和/或翻译后修饰。有趣的是，我们在一些硝酸纤维素水解点中也发现了细胞毒素和其他毒力因子，表明这些蛋白质可能在降低局部抗生素浓度中起作用。

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