...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Microbiology >Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection.
【24h】

Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection.

机译：基于非侵入性标本收集，实时RT-PCR在牛口蹄疫和猪口蹄疫以及经典猪瘟中的诊断特异性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Foot-and-mouth disease virus (FMDV) and classical swine fever virus (CSFV) are highly contagious and can cause great economic losses when introduced into disease-free regions. Accurate estimates of diagnostic specificity (Sp) are important when considering the implementation of surveillance for these agents. The purpose of this study was to estimate diagnostic Sp of a real-time reverse-transcriptase PCR assay developed for detection of FMDV in cattle and domestic swine and CSFV in domestic swine based on non-invasive specimen collection. One thousand and eighty-eight range beef cattle were sampled from thirteen geographic locations throughout Texas. One thousand and one hundred market hogs and cull sows were sampled. Results for both FMDV and CSFV were considered positive if amplification occurred at or before 40 PCR cycles, inconclusive between 40 and 45 cycles and negative otherwise. Ten cattle had nonspecific PCR amplifications for FMDV, but none were classified as positive and only one as inconclusive. Specificity (95% confidence interval) was estimated as 100% (99.7, 100). There were 19 nonspecific PCR amplifications for FMDV in sampled swine with 1 classified as positive, 6 as inconclusive, and 12 as negative. Specificity (95% confidence interval) was estimated as 99.9% (99.5, 100). There were 21 nonspecific PCR amplifications for CSFV, and 1 was classified as positive. Specificity (95% confidence interval) was estimated as 99.9% (99.5, 100). These assays have high Sp, but nonspecific PCR amplifications can occur.
机译：口蹄疫病毒（FMDV）和经典猪瘟病毒（CSFV）具有高度传染性，将其引入无病地区会造成巨大的经济损失。在考虑对这些药物实施监视时，准确估计诊断特异性（Sp）非常重要。这项研究的目的是基于非侵入性标本收集方法，评估用于实时检测牛和家猪FMDV和家猪CSFV的实时逆转录酶PCR检测方法的诊断Sp。从得克萨斯州的13个地理位置采样了188个肉牛。抽取了110只市场猪和公猪。如果扩增在40个PCR周期之前或之前进行，则FMDV和CSFV的结果均被视为阳性，在40至45个周期之间未得出结论，否则为阴性。 10头牛对FMDV进行了非特异性PCR扩增，但均未分类为阳性，只有1例没有定论。特异性（95％置信区间）估计为100％（99.7，100）。在采样猪中有19种针对FMDV的非特异性PCR扩增，其中1种归为阳性，6种为不确定，12种为阴性。特异性（95％置信区间）估计为99.9％（99.5，100）。 CSFV有21种非特异性PCR扩增，其中1种被归为阳性。特异性（95％置信区间）估计为99.9％（99.5，100）。这些测定具有很高的Sp，但可以发生非特异性PCR扩增。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号