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首页> 外文期刊>Veterinary Microbiology >Antimicrobial resistance and presence of virulence factor genes in Arcanobacterium pyogenes isolated from the uterus of postpartum dairy cows
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Antimicrobial resistance and presence of virulence factor genes in Arcanobacterium pyogenes isolated from the uterus of postpartum dairy cows

机译：从产后奶牛子宫分离的化脓性产弧菌中的抗菌素耐药性和致病因子基因的存在

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Arcanobacterium pyogenes is considered the most significant bacterium involved in the pathogenesis of metritis in cattle. Infections caused by antimicrobial-resistant bacteria are a great challenge in both human and veterinary medicine. The purpose of this study was to present an overview of antimicrobial resistance in A. pyogenes isolated from the uteruses of postpartum Holstein dairy cows and to identify virulence factors. Seventy-two A. pyogenes isolates were phenotypically characterized for antimicrobial resistance to amoxicillin, ampicillin, ceftiofur, chloramphenicol, florfenicol, oxytetracycline, penicillin, spectinomycin, streptomycin and tetracycline by the broth microdilution method. Presence of virulence factor genes of A. pyogenes was investigated. Isolates exhibited resistance to all antimicrobial agents tested; high levels of resistance were found to amoxicillin (56.9%); ampicillin (86.1%), chloramphenicol (100%), florfenicol (59.7%), oxytetracycline (54.2%), penicillin (86.1%) and tetracycline (50%). Of all isolates, 69(95.8%) were resistant to at least 2 of the antimicrobial agents tested and multidrug resistance (resistant to at least 3 antimicrobials) was observed in 64(88.9%) of the A. pyogenes isolates. The major multidrug resistance profile was found for chloramphenicol-ampicillin-penicillin-florfenicol-amoxicillin-tetracycline, which was observed in 21(29.2%) multidrug resistant isolates. No isolate was resistant to all nine antimicrobial agents tested but four isolates (5.6%) were resistant to eight antimicrobials. The information highlights the need for prudent use of specific antimicrobial agents. All four virulence factor genes occurred in isolates from normal puerperium and clinical metritis; however, the fimA gene was present in significantly higher frequency in isolates from metritis cows. (c) 2010 Elsevier B.V. All rights reserved.
机译：化脓性产弧菌被认为是与牛子宫炎的发病机理有关的最重要的细菌。由抗药性细菌引起的感染在人类和兽医学中都是巨大的挑战。这项研究的目的是概述分离自产后荷斯坦奶牛子宫的化脓性链球菌的抗药性，并确定其致病因子。通过肉汤微量稀释法对72个化脓农杆菌进行了表型鉴定，以证明对阿莫西林，氨苄青霉素，头孢噻呋，氯霉素，氟苯尼考，土霉素，青霉素，大观霉素，链霉素和四环素具有抗药性。研究了化脓性链球菌毒力因子基因的存在。分离株对所有测试的抗菌剂均显示出抗药性;发现对阿莫西林有高水平的耐药性（56.9％）;氨苄西林（86.1％），氯霉素（100％），氟苯尼考（59.7％），土霉素（54.2％），青霉素（86.1％）和四环素（50％）。在所有分离株中，有69（95.8％）对测试的至少两种抗菌剂有耐药性，在64（88.9％）个化脓性链球菌分离物中观察到了多药耐药性（对至少3种抗菌剂有耐药性）。在氯霉素-氨苄青霉素-青霉素-氟苯尼考-阿莫西林-四环素中发现了主要的多药耐药性，在21种（29.2％）多药耐药菌株中观察到。没有一种分离物对所有测试的九种抗菌药物都有抗药性，但是有四种分离物（5.6％）对八种抗药性有抗药性。该信息强调需要谨慎使用特定的抗菌剂。所有四个毒力因子基因均发生在正常产褥期和临床子宫炎的分离物中。但是，fimA基因在子宫炎牛分离株中的出现频率明显更高。 （c）2010 Elsevier B.V.保留所有权利。

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