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首页> 外文期刊>Veterinary Microbiology >Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests.
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Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests.

机译：OIE国际规定的血清学测试对源自特定ASF病毒基因型的新型非洲猪瘟病毒（ASF）抗体检测技术的比较评估。

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摘要

The presence of antibodies against African swine fever (ASF), a complex fatal notifiable OIE disease of swine, is always indicative of previous infection, since there is no vaccine that is currently used in the field. The early appearance and subsequent long-term persistence of antibodies combined with cost-effectiveness make antibody detection techniques essential in control programmes. Recent reports appear to indicate that the serological tests recommended by the OIE for ASF monitoring are much less effective in East and Southern Africa where viral genetic and antigenic diversity is the greatest. We report herein an extensive analysis including more than 1000 field and experimental infection sera, in which the OIE recommended tests are compared with antigen-specific ELISAs and immuno-peroxidase staining of cells (IPT). The antibody detection results generated using new antigen-specific tests, developed in this study, which are based on production of antigen fractions generated by infection and virus purification from COS-1 cells, showed strong concordance with the OIE tests. We therefore conclude that the lack of success is not attributable to antigenic polymorphism and may be related to the specific characteristics of the local breeds African pigs.
机译：抗非洲猪瘟（ASF）抗体的存在，这是一种复杂的致命可报告的OIE猪疾病，总是表明以前有过感染，因为该领域目前没有使用疫苗。抗体的早期出现和随后的长期持久性，再加上成本效益，使得抗体检测技术在控制程序中至关重要。最近的报道似乎表明，OIE推荐的用于ASF监测的血清学检测在病毒遗传和抗原多样性最大的东部和南部非洲效果要差得多。我们在此报告了广泛的分析，包括1000多个现场和实验感染血清，其中OIE建议的检测与抗原特异性ELISA和细胞免疫过氧化物酶染色（IPT）进行了比较。使用本研究开发的新抗原特异性测试产生的抗体检测结果，是基于从COS-1细胞感染和病毒纯化产生的抗原级分的产生，显示出与OIE测试的高度一致性。因此，我们得出的结论是，缺乏成功并非归因于抗原多态性，而可能与当地品种非洲猪的特定特征有关。

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