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首页> 外文期刊>Veterinarni Medicina >Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm
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Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm

机译：山羊繁殖场出现耐甲氧西林金黄色葡萄球菌的菌株

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摘要

The aim of this study was to report the detection of methicillin-resistant Staphylococcus aureus (MRSA) strains at a veterinary university goat breeding farm and their characteristics. A total of 278 samples collected from animals, milk, environment and farm personnel between June 2006 and March 2008 were examined. The identification of S. aureus isolates was performed by a species specific PCR assay. All detected isolates were tested for resistance to oxaeillin and other antimicrobials by phenotypicmethods and for the mecA gene by PCR method. Eight MRSA were detected in this study. Five of them originated from goat's milk and three were recovered from one human carrier of the farm personnel. All obtained MRSA isolates were clonally consistent andwere characterized as: SCCmec type IV, spa type t064, seb positive and for genes encoding TSST-1, PVL and exfoliative toxins A and B negative.
机译：这项研究的目的是报告在一家兽医大学的山羊育种场检测耐甲氧西林金黄色葡萄球菌（MRSA）菌株及其特征。在2006年6月至2008年3月之间，共对278份从动物，牛奶，环境和农场人员那里收集的样品进行了检查。金黄色葡萄球菌分离物的鉴定通过物种特异性PCR测定法进行。通过表型方法对所有检测到的分离物进行了对草霉素和其他抗菌剂的抗性测试，并通过PCR方法对了mecA基因进行了测试。在这项研究中检测到八个MRSA。其中有五种源自山羊奶，三种是从农场人员的一辆载具中回收的。所有获得的MRSA分离物在克隆上均一致，并被表征为：IV型SCCmec，t064型spa，seb阳性，编码TSST-1，PVL和脱落性毒素A和B的基因阴性。

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