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首页> 外文期刊>Veterinarni Medicina >Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using S900 real-time PGR: a case report
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Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using S900 real-time PGR: a case report

机译：鸟分枝杆菌亚种使用 S900实时PGR监测无足轻重症的穆福隆牧群中的副结核病：病例报告

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摘要

The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. This study followed the previous monitoring of the herd using a cultivation method which showed only a minimal infection load among the animals. Although no mouflon showed clinical symptoms, 35.7% and 80% of ewes were IS900-positive in 2008 and 2009, respectively. In seven out of 21 adult ewes, the presenceof the IS900 sequence was determined in 2008 as well as in 2009. Between the first and second sampling, twenty-three mouflon lambs born and kept with the ewes were examined. Almost one third of them (30.4%) were proven to have the MAP sequence in theirfaeces. Also, 75% environmental samples from the mouflon farm showed positivity. Infected animals without clinical symptoms which low sensitivity cultivation does not detect represent a source of infection for other animals. Therefore, real-time PCR hasa crucial role in paratuberculosis control programs, especially in control of the disease by the culling of infected animals.
机译：这项研究的目的是在两年多的时间里监测一个养殖的盘羊群中鸟分枝杆菌亚种的存在和持续存在。使用IS900实时PCR方法检测副结核病（MAP）。这项研究遵循了以前使用耕种方法对畜群进行监测的方法，该方法显示出动物之间的感染量很小。尽管没有mouflon表现出临床症状，但在2008年和2009年，分别有35.7％和80％的母羊IS900阳性。在2008年和2009年，在21头成年母羊中，有7头确定了IS900序列的存在。在第一次和第二次采样之间，检查了23头与母羊一起饲养的mouflon羔羊。其中近三分之一（30.4％）被证明在其粪便中具有MAP序列。此外，来自mouflon农场的75％环境样品显示出阳性。低灵敏度培养无法检测到的没有临床症状的感染动物代表了其他动物的感染源。因此，实时PCR在肺结核副反应的控制程序中，特别是在对被感染动物的剔除控制疾病中起着至关重要的作用。

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