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Fungal flora of normal eyes of healthy horses from the State of Rio de Janeiro, Brazil

机译:巴西里约热内卢州健康马的正常眼睛的真菌区系

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The conjunctival fungal flora of 32 adult horses with normal eyes (n = 64) from the State of Rio de Janeiro in Brazil was identified in the fall of 2000 using horses of different breeds, both genders and aged 5-19 years old. The culture samples were taken from the conjunctival sac of both eyes with a sterile cotton swab wetted with saline solution, seeded in Sabouraud's dextrose agar with chloramphenicol, and incubated for 5 days at an average temperature of 25 degreesC. The number of fungal colonies per eye varied between 0 and 250 colony forming units (CFUs). There were often differences in colony types between eyes of the same animal. Filamentous fungi of genera were isolated and identified in the following proportion of the total genera of fungal colonies isolated: Aspergillus (32.2%), Penicillium (25.8%), Scopulariopsis (15.9%), Trichoderma (11.2%), Cladosporium (5.6%), Mucor (2.1%), Syncephalastrum (2.1%), Eurotium (1.7%), Geotrichum (0.9%), Rhizopus (0.9%), Gliomastix (0.4%), Fusarium (0.4%), Staphylotrichum (0.4%) and Verticillium (0.4%). Yeast genera represented 9% of the total isolates. Over half the horses had at least one normal eye with either Aspergillus, Penicillium, Trichoderma or Scopulariopsis isolated, which is a departure from other studies of the normal horse eye.
机译:在2000年秋季,使用不同性别,年龄在5-19岁之间的马匹,鉴定了来自巴西里约热内卢州的32只眼睛正常(n = 64)的成年马的结膜真菌菌群。用无菌棉签用盐溶液浸湿从两只眼睛的结膜囊中取出培养物样品,将其接种到含有氯霉素的萨布罗氏葡萄糖琼脂中,并在25℃的平均温度下孵育5天。每只眼睛的真菌菌落数量在0到250个菌落形成单位(CFU)之间变化。同一只动物的眼睛之间的菌落类型通常存在差异。分离并鉴定出丝状真菌,占分离出的真菌菌落总数的以下比例:曲霉菌(32.2%),青霉菌(25.8%),鞘菌(15.9%),木霉菌(11.2%),枝孢菌(5.6%) ,Mucor(2.1%),前乳突肌(2.1%),((1.7%),土((0.9%),根霉菌(0.9%),胶质瘤(0.4%),镰刀菌(0.4%),葡萄球菌(0.4%)和黄萎病(0.4%)。酵母属占分离菌总数的9%。超过一半的马至少有一只正常眼,分离出曲霉菌,青霉菌,木霉菌或鞘吸虫,这与对正常马眼的其他研究有所不同。

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