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首页> 外文期刊>Veterinary Ophthalmology >Cellular-resolution in vivo imaging of the feline retina using adaptive optics: preliminary results.
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Cellular-resolution in vivo imaging of the feline retina using adaptive optics: preliminary results.

机译：利用自适应光学技术对猫视网膜进行细胞分辨率的体内成像：初步结果。

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Purpose: To perform cellular-level in vivo imaging of the feline retina using an adaptive optics flood illumination fundus camera (AO FIFC) designed for the human eye. Materials and methods: Cellular-level images were obtained from three eyes of two normal sedated cats. Ocular aberrations were corrected using an AO system based on a 52-acuator electromagnetic deformable mirror and a 1024 lenslet Hartmann-Shack sensor (both Imagine Eyes, Orsay, France). A square 3 degrees x 3 degrees area of the ocular fundus was flood-illuminated by a pulsed LED emitting at 850 nm and imaged onto a low-noise, high-resolution CCD camera. The animal's pupils were dilated and the effective pupil size was set to 7.5 mm. Conjunctival atraumatic clips were used to avoid eyeball movements and eyelid closure. The cornea was artificially hydrated throughout the experiments. Each acquisition consisted of 20 consecutive images, out of which 10 were numerically averaged to produce an enhanced final image. Results: The total amount of ocular aberrations was greatly reduced by the AO correction, from 2.4 to 0.21 microns root mean square on average. The resulting images presented white dots distributed at a density similar to that of cone photoreceptors and they allowed us to visualize small blood vessels and nerve fiber bundles at a higher resolution than classically obtained with conventional fundus photography. Conclusion: Retinal imaging with cellular resolution was feasible in cats under sedation using an AO FIFC designed for human eyes without any optical modification. The AO FIFC technology could find new applications in clinical, pharmacological, and toxicological investigations.Digital Object Identifier http://dx.doi.org/10.1111/j.1463-5224.2010.00829.x
机译：目的：使用专为人眼设计的自适应光学泛光照明眼底照相机（AO FIFC）对猫视网膜进行细胞水平的体内成像。材料和方法：从两只正常镇静的猫的三只眼获得细胞水平的图像。使用基于52针的电磁可变形镜和1024小透镜的Hartmann-Shack传感器（均为Imagine Eyes，法国奥赛）的AO系统校正眼像差。眼底的正方形3度x 3度区域被850 nm发射的脉冲LED泛光照明，并成像到低噪声，高分辨率CCD相机上。放大动物的瞳孔，并将有效瞳孔大小设置为7.5 mm。使用结膜无创夹避免眼球运动和眼睑闭合。在整个实验过程中将角膜人工水合。每次采集包括20个连续的图像，其中10个被数值平均以产生增强的最终图像。结果：通过AO校正，眼像差的总量大大减少，平均均方根从2.4微米降低到0.21微米。所产生的图像呈现出白点，其密度与圆锥形感光体的密度相似，并且使我们能够以比传统眼底照相法经典获得的更高的分辨率可视化小血管和神经纤维束。结论：使用专为人眼设计的AO FIFC，无需任何光学修饰，在镇静下对猫进行视网膜分辨率的细胞成像是可行的。 AO FIFC技术可以在临床，药理学和毒理学研究中找到新的应用。数字对象标识符http://dx.doi.org/10.1111/j.1463-5224.2010.00829.x

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