...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Parasitology >Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease
【24h】

Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease

机译：疑似tick传疾病的意大利犬血液中犬巴贝斯犬和巴贝斯犬的临床病理发现和DNA检测通过PCR-RFLP

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aims of this study were to determine the presence of Babesia spp. in blood samples from Italian dogs with clinical signs compatible with tick-borne diseases by means of PCR-restriction fragment length polymorphism (RFLP) and describe the clinicopathological findings of dogs with Babesia infection. We evaluated the majority of canine babesiosis cases by means of clinical history, physical examination, hematological, biochemical, serum electrophoresis, urinalysis and hemostatic tests. Forty-five out of 164 canine blood samples studied were positive to Babesia PCR-RFLP with the following results: Babesia canis canis (n=34) and Babesia canis vogeli (n=11). The majority of B. c. canis infections were detected in Northern Italy (29.1%; 30/103). B. c. vogeli cases were detected mainly in Central and Southern Italy (16.3%; 10/61). Only one B. c. vogeli was detected in Northern Italy (0.9%; 1/103). Three positive samples to B. c. canis and four positive samples to B. c. vogeli were selected for sequencing of a fragment of the 18S rRNA gene (410bp) for further molecular characterization. The sequence obtained from all seven dogs was 99/100% homologous to sequences from B. c. canis and B. c. vogeli, respectively, present in GenBank. Sixty-two percent of dogs infected with B. c. canis had recently travelled on a hunting trip to East European countries. The main acute clinical signs were dehydration, apathy, anorexia and fever. The majority of dogs infected with B. c. canis presented at initial clinical examination mild to severe thrombocytopenia, hyperfibrinogenemia, mild to moderate normocytic-normochromic non-regenerative anemia, hemolysis and neutropenia. The urinalysis showed hemoglobinuria in 13/19 dogs suggesting intravascular hemolysis. Dogs with B. c. canis infection had high levels of C-reactive protein. Hypoalbuminemia was present in 17/26 dogs. The 11 cases of B. c. vogeli infection did not present a homogenous clinicopathological pattern. B. c. vogeli infections were observed in young dogs causing hemolytic anemia and in adult/old does that frequently presented predisposing factors such as splenectomy or immunocompromised conditions. In conclusion, this study demonstrates the presence of B. c. canis and B. c. vogeli in Italian sick dogs and differences in clinicopathological pattern in these two species of B. canis.
机译：本研究的目的是确定巴贝虫属的存在。通过PCR限制性片段长度多态性（RFLP）从意大利犬的血液样本中检测出具有与tick传疾病兼容的临床体征的犬，并描述了巴贝虫感染犬的临床病理发现。我们通过临床病史，体格检查，血液学，生化，血清电泳，尿液分析和止血试验评估了大多数犬类贝贝病病例。在研究的164个犬血样本中，有45个对巴贝虫PCR-RFLP呈阳性，结果如下：犬贝贝菌（n = 34）和贝贝犬vogeli（n = 11）。大部分B. c。在意大利北部发现了犬科感染（29.1％; 30/103）。 c。主要在意大利中部和南部发现了vogeli病例（16.3％; 10/61）。仅一个B. c。在意大利北部发现了vogeli（0.9％; 1/103）。 B的三个阳性样本。 canis和B的四个阳性样本。选择vogeli对18S rRNA基因（410bp）的片段进行测序，以进行进一步的分子鉴定。从所有七只狗获得的序列与来自BC的序列的同源性为99/100％。卡尼斯和B. vogeli分别存在于GenBank中。百分之六十二的狗感染了B. c。 canis最近去了东欧国家打猎。主要的急性临床体征是脱水，冷漠，厌食和发烧。大部分被B.c.感染的狗初次临床检查时发现犬只存在轻度至重度血小板减少症，高纤维蛋白原性血，轻度至中度的正常性-正常性非再生性贫血，溶血和中性粒细胞减少。尿液分析显示13/19只狗出现血红蛋白尿，提示血管内溶血。与B.的狗犬感染具有高水平的C反应蛋白。低白蛋白血症存在于17/26只狗中。公元前11例vogeli感染未呈现均一的临床病理模式。 c。在引起溶血性贫血的幼犬中观察到了vogeli感染，在​​成年/成年犬中也观察到了vogeli感染，这经常表现出诸如脾切除术或免疫功能低下等诱发因素。总之，这项研究证明了B. c。的存在。犬和​​B.意大利生病犬中的vogeli以及这两种犬新孢子虫的临床病理模式差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号