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首页> 外文期刊>Veterinary Parasitology >Models for experimental infection of dogs fed with tissue from fetuses and neonatal cattle naturally infected with Neospora caninum.
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Models for experimental infection of dogs fed with tissue from fetuses and neonatal cattle naturally infected with Neospora caninum.

机译：用天然组织新孢子虫感染的胎儿和新生牛组织喂养的狗进行实验性感染的模型。

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摘要

Three models were designed to investigate the development and enteroepithelial phase of Neospora caninum in dogs, and to induce oocyst production by the parasite. In the first model, three dogs were fed raw fetal bovine tissue on two occasions. The bovine fetal tissue had been stored at 4 degrees C for 14 days and 16 days, respectively, and had tested positive for neosporosis using histopathology and immunohistochemistry. In the second model, nine dogs from the beginning of the experiment until euthanasia were fed portions of tissue from bovine fetuses that been stored at 4 degrees C for between 1 and 2 days. Three fetuses had tested positive for neosporosis using histopathology and immunohistochemistry. In the third model, three dogs were fed pieces of raw tissue from two neonatal calves that had not received colostrum, and which had tested positive for antibodies against N. caninum. The brains of these calves were positive for neosporosis by histopathology and immunohistochemistry. In all three models, none of the dogs excreted oocysts of N. caninum, developed intestinal parasites or seroconverted.
机译：设计了三种模型来研究犬新孢子虫的发育和肠上皮期，并诱导寄生虫产生卵囊。在第一个模型中，三只狗两次被喂食了原始胎牛组织。牛胎儿组织分别在4摄氏度下保存14天和16天，并使用组织病理学和免疫组织化学方法检测出新孢子虫病呈阳性。在第二种模型中，从实验开始到安乐死的9只狗被喂食了部分牛胎儿组织，这些组织在4摄氏度下储存了1至2天。使用组织病理学和免疫组织化学方法，三名胎儿的新孢子虫病呈阳性。在第三个模型中，给三只狗喂食了两只未初乳的新生小牛的原始组织，这些小牛的抗猪新孢子虫抗体呈阳性。通过组织病理学和免疫组织化学，这些小牛的大脑对新孢子虫病呈阳性。在所有三个模型中，没有狗排泄犬新孢子虫的卵囊，出现肠道寄生虫或发生血清转化。

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