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首页> 外文期刊>Virus Genes >Correlation between recombination junctions and RNA secondary structure elements in poliovirus Sabin strains.
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Correlation between recombination junctions and RNA secondary structure elements in poliovirus Sabin strains.

机译：脊髓灰质炎病毒萨宾菌株中重组连接和RNA二级结构元件之间的相关性。

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In order to test the hypothesis that RNA structural elements promote the distribution of certain types of recombination junctions in each one of the 2C and 3D poliovirus genomic regions (Sabin 3/Sabin 2 or Sabin 1 in 2C and Sabin 2/Sabin 1 or Sabin 3 in 3D), we searched in 2C and 3D regions of reference Sabin strains for high probability RNA structural elements that could promote recombination. Recombination junctions that were identified in clinical strains of this study, as well as in clinical strains of previous studies, were superimposed on RNA secondary structure models of 2C and 3D genomic regions. Furthermore, we created an in vitro model, based on double infection of cell-culture with two poliovirus strains, for the production and identification of recombinant Sabin strains in 2C and 3D regions. Our intention was to compare the results that refer to the correlation of recombination junctions and RNA secondary structures in 2C and 3D regions of clinical strains, with the respective results of the in vitro model. Most of the recombination junctions of the clinical strains were correlated with RNA secondary structure elements, which were identical between recombining Sabin strains, and also presented high predictive value. In consensus were, the respective results originated from the in vitro model. We propose that the distribution of specific types of recombination junctions in certain regions of Sabin strains is not fortuitous and is correlated with RNA secondary structure elements identical to both recombination partners. Furthermore, results of this study highlight an important role for the stem region of the RNA structure elements in promoting recombination.
机译：为了检验以下假设：RNA结构元件促进2C和3D脊髓灰质炎病毒基因组区域中的每一个（某些区域中的Sabin 3 / Sabin 2或Sabin 1以及2C中的Sabin 3 / Sabin 1或Sabin 3）促进某些类型的重组连接的分布在3D中），我们在参考Sabin菌株的2C和3D区域中搜索了可能促进重组的高概率RNA结构元件。在本研究的临床菌株以及先前研究的临床菌株中鉴定出的重组交界处被叠加在2C和3D基因组区域的RNA二级结构模型上。此外，我们基于两种脊髓灰质炎病毒株的细胞培养物的双重感染，创建了一个体外模型，用于在2C和3D区域生产和鉴定重组Sabin株。我们的目的是将涉及临床菌株2C和3D区域中重组连接和RNA二级结构相关性的结果与体外模型的相应结果进行比较。临床菌株的大多数重组连接点与RNA二级结构元件相关，这在重组萨宾菌株之间是相同的，并且也具有较高的预测价值。一致的是，各自的结果均源自体外模型。我们建议在萨宾菌株的某些区域中的特定类型的重组连接的分布不是偶然的，并且与与两个重组伙伴相同的RNA二级结构元素相关。此外，这项研究的结果突出了RNA结构元件的茎区域在促进重组中的重要作用。

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