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首页> 外文期刊>Virus Genes >Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus.
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Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus.

机译：中国烟草扭曲静脉病毒分离株的完整核苷酸序列和基因组组织。

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摘要

Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and disease symptoms were reproduced after experimental aphid transmission. The complete genome of TVDV was determined from cloned RT-PCR products derived from viral RNA. It was 5,920 nucleotides (nts) long and had the six major open reading frames (ORFs) typical of a member of the genus Polerovirus. Sequence comparisons showed that it differed significantly from any of the other species in the genus and this was confirmed by phylogenetic analyses of the RdRp and coat protein. SDS-PAGE analysis of purified virions gave two protein bands of about 26 and 59 kDa both of which reacted strongly in Western blots with antiserum produced to prokaryotically expressed TVDV CP showing that the two forms of the TVDV CP were the only protein components of the capsid.
机译：烟草丛枝顶病是由烟草丛枝顶病毒（TBTV，伞形病毒属的一种）引起的，该病毒依赖于烟草静脉扭曲病毒（TVDV）充当使TBTV衣壳化并使其通过蚜虫传播的辅助病毒。实验性蚜虫传播后，纯化了患病烟草植物的等距病毒粒子，并重现了疾病症状。从病毒RNA克隆的RT-PCR产物确定了TVDV的完整基因组。它长5,920个核苷酸（nts），并具有杆状病毒属成员典型的六个主要开放阅读框（ORF）。序列比较表明，它与该属中的任何其他物种均存在显着差异，这已通过对RdRp和外壳蛋白的系统发育分析得到证实。纯化病毒体的SDS-PAGE分析显示，两条蛋白带分别约26和59 kDa，在蛋白质印迹中均与产生于原核表达TVDV CP的抗血清强烈反应，显示出两种形式的TVDV CP是衣壳中唯一的蛋白成分。

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