...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Virus Genes >Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses.
【24h】

Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses.

机译：建立多重RT-PCR分析以检测猪H1和H3甲型流感病毒的不同谱系。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Classical swine H1N1, emerging European avian-like H1N1 and human-like H3N2 lineages are co-circulating in the swine population in China. The reverse transcriptase polymerase chain reaction (RT-PCR) assay is an effective method for use in influenza surveillance. In this study, a multiplex RT-PCR method was developed for simultaneous identification of hemagglutinin (HA) genes derived from the three lineages of swine influenza viruses. Three primer sets were designed and aimed specifically at HA genes of these viral lineages. The specificity of the assay showed that the established methods could efficiently differentiate the HA genes of classical swine H1N1, European avian-like H1N1, and human-like H3N2 viruses while other viruses such as classical swine fever virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, and porcine circovirus type 2, could not be detected. The assay showed a sensitivity of 1 x 10(2.5) 50% egg infectious dose for each virus lineage. The comparison of the results with those obtained from the analysis of 300 swine tracheal swab samples by means of virus isolation showed a high level of agreement. This multiplex RT-PCR method provides a rapid and specific swine influenza diagnostic tool that also has the potential for investigating the epidemiology of different lineages of swine influenza virus prevalent currently in China.
机译：经典猪H1N1，新兴的欧洲禽类H1N1和人类H3N2谱系在中国的猪群中共同传播。逆转录聚合酶链反应（RT-PCR）测定法是用于流感监测的有效方法。在这项研究中，开发了一种多重RT-PCR方法，用于同时鉴定源自猪流感病毒的三个谱系的血凝素（HA）基因。设计了三个引物组，并专门针对这些病毒谱系的HA基因。该方法的特异性表明，所建立的方法可以有效区分经典猪H1N1，欧洲禽样H1N1和人样H3N2病毒的HA基因，而其他病毒，如经典猪瘟病毒，猪繁殖和呼吸综合征病毒，无法检测到伪狂犬病病毒和2型猪圆环病毒。该检测结果显示，每种病毒谱系对鸡蛋感染剂量的敏感性为1 x 10（2.5）50％。结果的比较与通过病毒分离对300头猪气管拭子样品的分析所获得的结果显示出很高的一致性。这种多重RT-PCR方法提供了一种快速而特异性的猪流感诊断工具，也具有调查目前在中国流行的猪流感病毒不同谱系的流行病学的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号