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首页> 外文期刊>Virus Genes >Molecular characterization of a distinct begomovirus species from Vernonia cinerea and its associated DNA-beta using the bacteriophage Phi 29 DNA polymerase.
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Molecular characterization of a distinct begomovirus species from Vernonia cinerea and its associated DNA-beta using the bacteriophage Phi 29 DNA polymerase.

机译：使用噬菌体Phi 29 DNA聚合酶对灰质Vernonia cinerea及其相关的DNA-beta的独特的bemomovirus物种进行分子表征。

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Vernonia cinerea plants with yellow vein symptoms were collected around crop fields in Madurai. A portion (550 bp) of the AV1 gene amplified using degenerate primers from the total DNA purified from diseased leaf sample was cloned and sequenced. Specific primers derived from the above sequence were used to amplify 2,745 nucleotides with the typical genome organization of begomoviral DNA A (EMBL Accession No. AM182232). Sequence comparison with other begomoviruses revealed the greatest identity (82.4%) with Emilia yellow vein virus (EmYVV-[Fz1]) from China and less than 80% with all other known begomoviruses. The International Committee on Taxonomy of Viruses (ICTV) has therefore recognized Vernonia yellow vein virus (VeYVV) as a distinct begomovirus species. Conventional PCR could not amplify the DNA B or DNA beta from the diseased tissue. However, the beta DNA (1364 bp) associated with the disease was obtained (Accession No. FN435836) by the rolling circle amplification-restriction fragment length polymorphism method (RCA-RFLP) using Phi 29 DNA polymerase. Sequence analysis shows that DNA beta of VeYVV has the highest identity (56.8%) with DNA beta of Sigesbeckia yellow vein Guangxi betasatellite (SibYVGxB-[CN: Gx111:05]) and 56-53% with DNA beta associated with other begomoviruses. This is the first report of the molecular characterization of VeYVV from V. cinerea in India. The complete molecular characterization, phylogenetic analysis, and putative recombination events in VeYVV are reported.
机译：在马杜赖（Madurai）的田地周围收集了带有黄色静脉症状的灰粉病植物。从从患病叶片样品中纯化的总DNA中使用简并引物扩增的AV1基因的一部分（550 bp）被克隆并测序。来自上述序列的特异性引物被用于扩增具有前牛乳杆菌DNA A（EMBL登录号AM182232）的典型基因组结构的2,745个核苷酸。与其他begomovirus病毒的序列比较显示，与来自中国的艾米利亚黄静脉病毒（EmYVV- [Fz1]）的同一性最高（82.4％），而与所有其他已知begomovirus病毒的同一性不足80％。因此，国际病毒分类学委员会（ICTV）认可了Vernonia黄脉病毒（VeYVV）是一种独特的贝莫病毒。常规PCR不能从患病组织中扩增DNA B或DNAβ。然而，使用Phi 29 DNA聚合酶通过滚环扩增-限制性片段长度多态性方法（RCA-RFLP）获得了与疾病相关的βDNA（1364bp）（登录号FN435836）。序列分析显示，VeYVV的DNAβ与西格斯黄静脉广西beta卫星（SibYVGxB- [CN：Gx111：05]）的DNAβ具有最高的同一性（56.8％），与与其他begomovirus病毒相​​关的DNAβ的56-53％具有最高的同一性。这是印度V.cinerea的VeYVV分子表征的首次报道。 VeYVV中完整的分子表征，系统发育分析和推定的重组事件已报道。

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