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首页> 外文期刊>Virus Research: An International Journal of Molecular and Cellular Virology >Long-term RNA persistence of Porcine rubulavirus (PorPV-LPMV) after an outbreak of a natural infection: The detection of viral mRNA in sentinel pigs suggests viral transmission
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Long-term RNA persistence of Porcine rubulavirus (PorPV-LPMV) after an outbreak of a natural infection: The detection of viral mRNA in sentinel pigs suggests viral transmission

机译：天然感染爆发后猪风疹病毒（PorPV-LPMV）的长期RNA持久性：前哨猪中病毒mRNA的检测表明病毒传播

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摘要

The persistence of porcine rubulavirus (PorPV-LPMV) in five pigs that had survived an outbreak of a natural infection was determined. After the resolution of the outbreak, each animal was housed in an isolation pen together with one sentinel pig. Approximately every 2 months thereafter one group of animals was euthanized and tissue samples taken for virological and serological analysis. Infectious virus was not isolated from any samples; antibodies to PorPV-LPMV were detected in convalescent pigs by virus neutralisation test and blocking ELISA but not in sentinel pigs. PorPV-LPMV mRNA of the nucleoprotein (NP) and phosphoprotein (P) genes was detected by a nested polymerase chain reaction (nPCR) in samples of trigeminal and optic nerves, cervical spinal cord, tonsils, salivary gland, lung and pancreas from convalescent pigs. mRNA was also detected in the midbrain, corpus callosum, or olfactory bulb in four out of five pigs by nRT-PCR, this result was confirmed by the sequencing of a 260 bp PCR product of P gene region. The highest average viral copies/mu g of total RNA occurred in the olfactory bulb and pancreas tissues of convalescent pigs and midbrain, tonsil and pancreas of sentinel pigs housed with the convalescent pigs. Satellitosis and gliosis of the midbrain, olfactory bulb, corpus callosum, medulla oblongata or choroid plexus were microscopically observed in four convalescent pigs. The control pig remained negative in all tests. The results indicate that PorPV-LPMV mRNA persists and induces a durable humoral immune response in pigs that have recovered from a natural infection. After a possible reactivation of the virus, it was transmitted to sentinel pigs in contact with the convalescent pigs. (C) 2014 Elsevier B.V. All rights reserved.
机译：确定了在自然感染爆发后幸存的五只猪中的猪风疹病毒（PorPV-LPMV）的持久性。爆发爆发后，将每只动物与一只定点猪一起关在隔离栏中。此后大约每2个月对一组动物实施安乐死并取组织样品进行病毒学和血清学分析。没有从任何样品中分离出传染性病毒。通过病毒中和试验和封闭酶联免疫吸附试验（ELISA）在恢复期的猪中检测到针对PorPV-LPMV的抗体，但在前哨猪中未检出。通过巢式聚合酶链反应（nPCR）在con愈猪的三叉神经和视神经，颈脊髓，扁桃体，唾液腺，肺和胰腺样本中检测到了核蛋白（NP）和磷蛋白（P）基因的PorPV-LPMV mRNA 。通过nRT-PCR在5头猪中的4头中脑，call体或嗅球中也检测到了mRNA，通过对P基因区的260 bp PCR产物进行测序，证实了这一结果。最高的平均病毒拷贝/μg总RNA出现在恢复期猪的嗅球和胰腺组织以及与恢复期猪一起饲养的前哨猪的中脑，扁桃体和胰腺。在四个恢复期的猪中，显微镜下观察到中脑，嗅球，call体，长延髓或脉络丛的卫星化和神经胶质化。对照猪在所有测试中均保持阴性。结果表明，从自然感染中恢复过来的猪中，PorPV-LPMV mRNA持续存在并诱导持久的体液免疫反应。可能的病毒重新激活后，将其传播到与恢复期猪接触的前哨猪。 （C）2014 Elsevier B.V.保留所有权利。

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