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首页> 外文期刊>Pesquisa Veterinaria Brasileira >Identification of Staphylococcus strains isolated from bovine mastitis by PCR and 16S rDNA sequencingOriginal Title (non-English) Uso de PCR e sequenciamento do rDNA 16S para identificacao de bacterias do genero Staphylococcus isoladas de mastite bovina [Portuguese]
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Identification of Staphylococcus strains isolated from bovine mastitis by PCR and 16S rDNA sequencingOriginal Title (non-English) Uso de PCR e sequenciamento do rDNA 16S para identificacao de bacterias do genero Staphylococcus isoladas de mastite bovina [Portuguese]

机译：通过PCR和16S rDNA测序鉴定从牛乳腺炎中分离出的葡萄球菌菌株原始名称（非英语）使用PCR和16S rDNA测序鉴定从牛乳腺炎中分离出的葡萄球菌细菌[葡萄牙语]

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Lange C.C., Brito M.AV.P., Brito J.R.F., Arcuri E.F., Souza G.N., Machado M.A., Domingues R. & Salimena A.P.S. 2011. [Identification of Staphylococcus strains isolated from bovine mastitis by PCR and 16S rDNA sequencing.] Uso de PCR e sequenciamento do rDNA 16S para identificacao de bacterias do genero Staphylococcus isoladas de mastite bovina. Pesquisa Veterinaria Brasileira 31(1):36-40. Embrapa Gado de Leite, Rua Eugenio do Nascimento 610, Juiz de Fora, MG 36030-330, Brazil. E-mail: clange@cnpgl.embrapa.brThe objective of this study was to identify the species of 100 isolates of Staphylococcus from mastitis in dairy cows from herds located in the state of Minas Gerais, Brazil. PCR reactions were carried out using specific primers described previously for S. aureus (femA gene), S. intermedius (16S rDNA) and S. hyicus (16S-23S rDNA spacer region). In addition, products of amplification of variable regions of the 16S rDNA gene of the strains were sequenced. According to the results of the PCR, 83 strains were identified as S. aureus, 13 as S. intermedius, two as S. hyicus and two isolates were not identified. The sequencing of 16S rDNA was applied to 23 strains identified by PCR amplifications: six S. aureus and the strains identified as S. intermedius (n=13), S. hyicus (n=2) or not identified (n=2). The sequencing of 16S rDNA confirmed the six strains as S. aureus. The others 17 strains were identified as S. chromogenes (13 isolates) and S. hyicus (four isolates). Each sample was related to a specie according to the smallest E-value and highest similarity (>= 99%). The identification of S. hyicus and S. chromogenes was accomplished only by 16S rDNA sequencing.
机译：Lange C.C.，Brito M.AV.P.，Brito J.R.F.，Arcuri E.F.，Souza G.N.，Machado M.A.，Domingues R.和Salimena A.P.S. 2011. [通过PCR和16S rDNA测序鉴定从牛乳腺炎中分离出的葡萄球菌菌株。]超脱氧核糖核酸测序后的rDNA的16S副细菌鉴定出的葡萄球菌是普通葡萄球菌。 Pesquisa Veterinaria Brasileira 31（1）：36-40。 Embrapa Gado de Leite，Ru Eugenio do Nascimento 610，Juiz de Fora，MG 36030-330，巴西。电子邮件：clange@cnpgl.embrapa.br这项研究的目的是鉴定位于巴西米纳斯吉拉斯州的奶牛场中，乳牛炎中葡萄球菌的100株分离菌的种类。使用先前针对金黄色葡萄球菌（femA基因），中间链球菌（16S rDNA）和葡萄球菌（16S-23S rDNA间隔区）描述的特异性引物进行PCR反应。另外，对菌株的16S rDNA基因的可变区的扩增产物进行了测序。根据PCR结果，鉴定出83株为金黄色葡萄球菌，13株为中间链球菌，2株为猪链球菌，2株分离株。将16S rDNA的测序应用于通过PCR扩增鉴定的23个菌株：6个金黄色葡萄球菌以及鉴定为中间链球菌（n = 13），葡萄球菌（n = 2）或未鉴定（n = 2）的菌株。对16S rDNA的测序证实了这6个菌株为金黄色葡萄球菌。其他17个菌株被鉴定为发色链球菌（13个分离株）和猪链球菌（4个分离株）。根据最小的E值和最高的相似性（> = 99％），每个样本都与一个物种相关。葡萄球菌和发色链球菌的鉴定仅通过16S rDNA测序即可完成。

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