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Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus)

机译:新西兰兔的泪液产生,眼压和结膜微生物群,细胞学和组织学

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摘要

The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora conjunctival cytology and conjunctival histology were also performed. Mean (+/- SD) for STT1, EAPPTTa, EAPPTTb and LOP was 7.27 +/- 2.51mm/min, 12.43 +/- 1.69mm/min, 15.24 +/- 2.07mm/min, 12.89 +/- 2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes.
机译:这项研究的目的是为新西兰兔(Oryctolagus cuniculus)的眼科诊断测试建立参考值。总共使用了22只成年雄性兔子。眼科测试包括使用两种不同的商业品牌材料通过Schirmer泪液测试1(STT1)和牙髓吸收纸点泪液测试(EAPPTT)评估泪液产生。压平眼压测定法,结膜细菌菌群的培养,结膜细胞学和结膜组织学检查。 STT1,EAPPTTa,EAPPTTb和LOP的平均值(+/- SD)为7.27 +/- 2.51mm / min,12.43 +/- 1.69mm / min,15.24 +/- 2.07mm / min,12.89 +/- 2.80mm Hg , 分别。表皮葡萄球菌,葡萄球菌。和芽孢杆菌占主导地位。细胞学评估显示存在柱状上皮细胞,浅层鳞状角化细胞,淋巴细胞,异嗜性,红细胞,黏液和细菌。组织学分析显示分层的上皮,其特征在于存在具有大量杯状细胞的柱状上皮细胞。报告的数据可用于治疗或实验目的。

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