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首页> 外文期刊>Pesquisa Veterinaria Brasileira >Use of third eyelid and rectal mucosa biopsies for diagnosis of sheep scrapie on a farm in southern Brazil. [Portuguese]Original Title Utilizacao de biopsias da terceira palpebra e mucosa retal em ovinos para diagnostico de scrapie em uma propriedade da regiao sul do Brasil.
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Use of third eyelid and rectal mucosa biopsies for diagnosis of sheep scrapie on a farm in southern Brazil. [Portuguese]Original Title Utilizacao de biopsias da terceira palpebra e mucosa retal em ovinos para diagnostico de scrapie em uma propriedade da regiao sul do Brasil.

机译：在巴西南部的一个农场中，使用第三眼睑和直肠粘膜活组织检查诊断绵羊瘙痒病。 [葡萄牙语]原标题在巴西南部一处物业的绵羊第三眼睑和直肠粘膜活检组织中用于诊断瘙痒病。

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Scrapie, a form of transmissible spongiform encephalopathy (TSEs) is a fatal neurodegenerative disorder that affects sheep and goats. The disease is characterized by an accumulation of the abnormal prionic protein (PrPSc) in the encephalic and lymphoreticular tissues. This paper describes the use of anti-prionic protein immunohistochemical (IHC) procedure as a method of pre-clinical diagnosis of scrapie.The test was carried out in biopsied lymphoreticular tissues from third eyelid and rectal mucosa. Anti-prion protein monoclonal antibodies F89/160.1.5 and F99/97.6.1 were used. Scrapie diagnosis in lymphoreticular tissues through IHC was achieved when the samples had a minimum of three lymphoid follicles in well delimited germinal centre. Positive immunostaining was identified in 19 out of 318 samples of the third eyelid. Material sampled at post-mortem examination in 18 of these scrapie-positive sheep, which were previously verified by biopsy, and in 21 of its relatives, was confirmed with IHC tests. Positive immunostaining from rectal mucosa tissue was not observed. Third eyelid and tonsil were the organs with the larger amount of positive immunostaining (18/18 and 8/18 respectively) at post-mortem examination. None positive result was obtained along the 21 animals related to the positive ones, and none of the positive cases showed IHC labeling in the brain. The use of lymphoid tissues for scrapie diagnosis by IHC through biopsies showed to be a viable and efficient method for pre-clinical diagnostic.
机译：瘙痒病是一种可传播的海绵状脑病（TSE），是一种致命的神经退行性疾病，会影响绵羊和山羊。该病的特征是在脑和淋巴网状组织中积累了异常的prionic蛋白（PrP Sc ）。本文描述了抗普利诺蛋白免疫组织化学（IHC）程序作为刮伤的临床前诊断方法的方法。该测试在活检的第三眼睑和直肠粘膜的淋巴网状组织中进行。使用了抗-病毒蛋白单克隆抗体F89 / 160.1.5和F99 / 97.6.1。当样品在明确界定的生发中心至少有三个淋巴滤泡时，可通过IHC对淋巴网状组织进行刮擦诊断。在318个第三眼皮样本中，有19个被鉴定为阳性免疫染色。在尸检后对其中18只羊的阳性羊进行了尸检，这些羊先前已通过活组织检查得到了验证，其亲属中的21只已经通过IHC测试得到了确认。没有观察到来自直肠粘膜组织的阳性免疫染色。验尸后，第三眼睑和扁桃体是阳性免疫染色较多的器官（分别为18/18和8/18）。在与阳性动物相关的21只动物中未获得阳性结果，并且没有阳性病例在大脑中显示IHC标记。淋巴组织用于活检组织通过活检诊断瘙痒病的方法被证明是临床前诊断的可行和有效方法。

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