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首页> 外文期刊>Pesquisa Veterinaria Brasileira >Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Parana state, Brazil
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Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Parana state, Brazil

机译：巴西巴拉那州圈养的野猪（Sus scrofa scrofa）中猪细小病毒，猪细小病毒和肠病毒G的分子调查

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摘要

Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric picornaviruses in wild boars (Sus scrofa scrofa) of Parana state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Parana state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5'non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87.1% to 100% for EV-G. Peccaries tested negative for the viruses and in this study they did not represent infection reservoirs. This study is the first to report the molecular detection of PTV, PSV, and EV-G from captive wild boars in a South American country and the first to screen peccaries as alternative host species for porcine enteric picornavirus.
机译：猪破伤风病毒（PTV），猪细小病毒（PSV）和肠病毒G（EV-G）是特有的猪宿主物种的传染病原体，在世界各地广泛传播。这项研究旨在调查这些猪肠道小核糖核酸病毒在巴西巴拉那州的野猪（Ss scrofa scrofa）中的自然感染，并评估作为这些病毒的替代宿主物种的野猪（Pecari tajacu和Tayassu pecari）。来自无症状野猪（n = 22）的粪便样本（n = 36），年龄在2至7个月大（年轻，n = 14）和2至4岁（成人，n = 8），且来自野猪（6到8个月大时，n = 14）分别来自都位于巴拉那州的一个农场和一个动物园。靶向病毒基因组5'非翻译区的逆转录-聚合酶链反应（RT-PCR）和巢式PCR（n-PCR）分析用于筛选病毒。在22份野猪粪便样本中的12份中检出了猪肠道小核糖核酸病毒。根据每种病毒，检测到EV-G的频率最高（11 / 22，50％），其次是PTV（10 / 22，45.5％）和PSV（4 / 22，18.2％）。关于年龄组，与成年动物（3 / 8，37.4％）相比，年轻野猪感染PTV，PSV和EV-G的频率更高（9 / 14，64.3％）。将每种病毒的一种n-PCR扩增产物进行测序分析，并将核苷酸序列与相关病毒进行比较，结果显示相似性从PTV的97.7％至100％，PSV的92.4％至96.2％不等，以及87.1 EV-G为％到100％。真菌对病毒的检测结果为阴性，在本研究中，它们不代表感染库。这项研究是第一个报告从南美一个国家的圈养野猪中检测PTV，PSV和EV-G的分子，也是第一个筛选出作为猪肠道小核病毒替代宿主物种的野猪。

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