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首页> 外文期刊>The International journal of oral & maxillofacial implants >Effects of titanium surface roughness on mesenchymal stem cell commitment and differentiation signaling.
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Effects of titanium surface roughness on mesenchymal stem cell commitment and differentiation signaling.

机译：钛表面粗糙度对间充质干细胞定型和分化信号的影响。

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PURPOSE: Human mesenchymal stem cells (hMSCs) are primary cells capable of differentiating to osteocytic lineage when stimulated under appropriate conditions. This study examined changes in hMSC morphology, proliferation, and gene expression after growth on machined or dual acid-etched (AE) titanium surfaces. MATERIALS AND METHODS: hMSCs, isolated from adult human bone marrow, were cultured on titanium surfaces. The two specimens of titanium surfaces in this study included machined and AE titanium disks. Cell morphology was evaluated by scanning electron microscopy, and cell proliferation and collagen synthesis were estimated by measuring the amount of 3H-thymidine incorporation into DNA and 3H-proline incorporation into collagen fibers. Alkaline phosphatase (ALP) activity was determined by measuring the release of p-nitrophenol from disodium p-nitrophenyl phosphate. Changes in gene expression for bone morphogenetic protein-2 (BMP-2), Runx2 type II, Osterix (Osx), osteopontin, type I collagen, ALP, osteocalcin, and bone sialoprotein were determined by reverse-transcriptase polymerase chain reaction after 22 days of in vitro culture in osteogenic medium. RESULTS: The two substrates had no significant effects on cell adhesion and proliferation. Morphologic characteristics were observed by scanning electron microscopy. hMSCs on the machined surface spread more and were flatter than cells cultured on the AE surface. Osteopontin mRNA expression was similar on all surfaces, and the other mRNA transcripts were increased in hMSC cultured on AE surface. In particular, BMP-2, Runx2, and Osx, three osteogenic factors that induce the progressive differentiation of multipotent mesenchymal cells into osteoblasts, were expressed more on AE titanium than on machined titanium. Collagen and ALP assays confirmed the highest level of mRNA transcripts correlated with increases in these proteins. CONCLUSION: These results showed that an AE titanium surface stimulated the expression of markers of osteoblastic phenotype more than a machined titanium surface.
机译：目的：人间充质干细胞（hMSCs）是在适当条件下刺激后能够分化为骨细胞系的原代细胞。这项研究检查了在机械或双酸蚀（AE）钛表面上生长后hMSC形态，增殖和基因表达的变化。材料与方法：从成年人骨髓中分离出的hMSCs在钛表面上培养。这项研究中的两个钛表面样本包括机加工和AE钛盘。通过扫描电子显微镜评估细胞形态，并通过测量3H-胸苷掺入DNA和3H-脯氨酸掺入胶原纤维的量来估计细胞增殖和胶原合成。通过测量对硝基苯磷酸二钠钠中对硝基苯酚的释放来确定碱性磷酸酶（ALP）的活性。 22天后通过逆转录酶聚合酶链反应确定骨形态发生蛋白2（BMP-2），Runx2 II型，Osterix（Osx），骨桥蛋白，I型胶原，ALP，骨钙蛋白和骨唾液蛋白基因表达的变化。成骨培养基的体外培养结果：两种底物对细胞的黏附和增殖均无明显影响。通过扫描电子显微镜观察形态学特征。与在AE表面上培养的细胞相比，在经过加工的表面上的hMSC分布更广，并且更平坦。在AE表面培养的hMSC中，骨桥蛋白mRNA表达在所有表面上均相似，而其他mRNA转录物则有所增加。尤其是，BMP-2，Runx2和Osx是诱导多能间充质细胞逐渐分化为成骨细胞的三种成骨因子，它们在AE钛上的表达高于在机加工钛上的表达。胶原蛋白和ALP分析证实了与这些蛋白质增加相关的最高水平的mRNA转录物。结论：这些结果表明，AE钛表面比机械加工的钛表面更能刺激成骨细胞表型标志物的表达。

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