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首页> 外文期刊>Virology >The Epstein-Barr virus transactivator Zta binds to its own promoter and is required for full promoter activity during anti-Ig and TGF-beta1 mediated reactivation
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The Epstein-Barr virus transactivator Zta binds to its own promoter and is required for full promoter activity during anti-Ig and TGF-beta1 mediated reactivation

机译：爱泼斯坦-巴尔病毒反式激活因子Zta与其自身的启动子结合，是抗Ig和TGF-beta1介导的再激活过程中完整启动子活性所必需的

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摘要

Transcription of the immediate early gene BZLF1 is mediated initially through the activation of cellular transcription factors. Reporter-based studies have provided evidence that following this initial activation, the BZLF1 gene product Zta may be involved in an autoactivation loop through binding to its promoter Zp. In contrast, other reports have shown that transfection of a Zta expression vector in latently infected cells does not activate endogenous Zp. Using chromatin immunoprecipitation (ChIP) assays, we show here that Zta binds to endogenous Zp following induction of the lytic cycle by anti-Ig and TGF-beta 1 and that binding occurs early enough to play a role in the activation of Zp. We have also generated a dominant-negative Zta and shown that it inhibits activation of endogenous Zp. These data support a two-step model for Zp activation during reactivation involving initial activation by cellular factors followed by an autoactivation step. (C) 2004 Elsevier Inc. All rights reserved.
机译：立即早期基因BZLF1的转录最初是通过激活细胞转录因子来介导的。基于记者的研究提供了证据，表明在此初始激活后，BZLF1基因产物Zta可能通过与其启动子Zp结合而参与自激活环。相反，其他报道表明在潜在感染的细胞中转染Zta表达载体不会激活内源性Zp。使用染色质免疫沉淀（ChIP）分析，我们在这里显示Zta与内源性Zp结合，之后是由抗Ig和TGF-beta 1诱导的裂解周期，并且这种结合发生得足够早，可以在Zp的激活中发挥作用。我们还生成了一个显性负Zta，并表明它抑制了内源性Zp的激活。这些数据支持在重激活过程中Zp激活的两步模型，涉及通过细胞因子进行的初始激活以及随后的自动激活步骤。 （C）2004 Elsevier Inc.保留所有权利。

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