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首页> 外文期刊>Virology >A more precise HIV integration assay designed to detect small differences finds lower levels of integrated DNA in HAART treated patients.
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A more precise HIV integration assay designed to detect small differences finds lower levels of integrated DNA in HAART treated patients.

机译：旨在检测微小差异的更精确的HIV整合测定法可发现HAART治疗患者的整合DNA水平较低。

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摘要

Many studies report the level of total viral DNA in HIV-infected patients, but few studies report the level of integrated DNA. It is important to measure integrated DNA in HIV-infected patients because the information could shed light on the effectiveness of antiretroviral therapy, especially intensified therapy, when viral loads may remain undetectable. In order to develop an integration assay for patient samples, we enhanced the sensitivity of our prior integration assay. To do this, we exploited a technique that we developed, called repetitive sampling, and optimized reaction conditions for rare event detection, rather than large dynamic range. We also designed our primers to match more conserved regions of HIV. The result is a new, sensitive, quantitative assay that allows us to measure integrated DNA in HIV-infected patients. When we applied our integration assay to patient PBMCs, we found that the use of HAART is associated with reduced levels of integrated DNA.
机译：许多研究报告了HIV感染患者的总病毒DNA水平，但很少有研究报告整合的DNA水平。在感染HIV的患者中测量整合DNA的重要性非常重要，因为当病毒载量可能仍无法检测到时，该信息可能会揭示抗逆转录病毒疗法（尤其是强化疗法）的有效性。为了开发用于患者样品的整合测定，我们提高了先前整合测定的灵敏度。为此，我们开发了一种称为重复采样的技术，并针对稀有事件检测（而非较大的动态范围）优化了反应条件。我们还设计了引物以匹配更保守的HIV区域。结果是一种新颖，灵敏，定量的测定方法，使我们能够测量HIV感染患者的整合DNA。当我们将整合测定应用于患者PBMC时，我们发现HAART的使用与整合DNA含量降低相关。

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