• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Virology >The epithelial-mesenchymal transition induced by keratinocyte growth conditions is overcome by E6 and E7 from HPV16, but not HPV8 and HPV38: characterization of global transcription profiles.
【24h】

The epithelial-mesenchymal transition induced by keratinocyte growth conditions is overcome by E6 and E7 from HPV16, but not HPV8 and HPV38: characterization of global transcription profiles.

机译：角质形成细胞生长条件诱导的上皮-间质转化被HPV16的E6和E7克服了，但HPV8和HPV38却无法克服：全局转录谱的表征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to evaluate the growth properties of primary human keratinocytes expressing E6 and E7 proteins, which are from either the beta- or alpha-genotypes, under different culture conditions. We demonstrated that keratinocytes expressing E6 and E7, from both HPV8 and 38, irreversibly underwent the epithelial-mesenchymal transition (EMT) when grown on plastic with FAD medium (F12/DMEM/5%FBS). Expression of E6/E7 from HPV16 was capable of fully overcoming the FAD-induced EMT. Immortalization was only observed in HPV16-transduced cell lines, while the more proliferating phenotype of both KerHPV8 and 38 was mainly related to FAD-induced EMT. Microarray analysis of exponentially growing cells identified 146 cellular genes that were differentially regulated in HPV16 compared to HPV8- and 38-transduced cells. A large accumulation of transcripts associated with epidermal development and differentiation was observed in HPV16-transduced cells, whereas transcripts of genes involved in the extracellular matrix, multicellular organismal processes, and inflammatory response were affected in HPV8 and 38-transduced cells.
机译：这项研究的目的是评估在不同的培养条件下表达E6和E7蛋白的原代人类角质形成细胞的生长特性，这些蛋白来自β型或α型。我们证明了当在带有FAD培养基（F12 / DMEM / 5％FBS）的塑料上生长时，从HPV8和38表达E6和E7的角质形成细胞不可逆地经历了上皮-间质转化（EMT）。 HPV16中E6 / E7的表达能够完全克服FAD诱导的EMT。永生化仅在HPV16转导的细胞系中观察到，而KerHPV8和38的更增殖表型主要与FAD诱导的EMT相关。对呈指数增长的细胞进行的微阵列分析确定了与HPV8和38转导的细胞相比，在HPV16中差异调节的146个细胞基因。在HPV16转导的细胞中观察到大量与表皮发育和分化相关的转录本，而在HPV8和38转导的细胞中，参与细胞外基质，多细胞生物过程和炎症反应的基因的转录本受到影响。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号