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首页> 外文期刊>Virology >Isolation and characterization of Solenopsis invicta virus 3, a new positive-strand RNA virus infecting the red imported fire ant, Solenopsis invicta.
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Isolation and characterization of Solenopsis invicta virus 3, a new positive-strand RNA virus infecting the red imported fire ant, Solenopsis invicta.

机译：贪食贪食菌3的分离和鉴定，它是一种新型正链RNA病毒，可感染红色的进口火蚁贪食贪食菌。

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摘要

We report the discovery of a new virus from the red imported fire ant, Solenopsis invicta. Solenopsis invicta virus 3 (SINV-3) represents the third virus discovered from this ant species using the metagenomics approach. The single (positive)-strand RNA, monopartite, bicistronic genome of SINV-3 was sequenced in entirety (GenBank accession number FJ528584), comprised of 10,386 nucleotides, and polyadenylated at the 3' terminus. This genome size was confirmed by Northern analysis. The genome revealed 2 large open reading frames (ORFs) in the sense orientation with an untranslated region (UTR) at each end and between the two ORFs. The 5' proximal ORF (ORF 1) encoded a predicted protein of 299.1 kDa (2580 amino acids). The 3' proximal ORF (ORF 2) encoded a predicted protein of 73.2 kDa (651 amino acids). RNA-dependent RNA polymerase (RdRp), helicase, and protease domains were recognized in ORF 1. SDS-PAGE separation of purified SINV-3 particles yielded 2 bands (ostensibly capsid proteins) with a combined molecular mass of 77.3 kDa which was similar to the mass predicted by ORF 2 (73.2 kDa). Phylogenetic analysis of the conserved amino acid sequences containing domains I to VIII of the RdRp from dicistroviruses, iflaviruses, plant small RNA viruses, picornaviruses, and 4 unassigned positive-strand RNA viruses revealed a trichotomous phenogram with SINV-3 and Kelp fly virus comprising a unique cluster. Electron microscopic examination of negatively stained samples of SINV-3 revealed isometric particles with apparent projections and a diameter of 27.3+/-1.3 nm. SINV-3 was successfully transmitted to uninfected workers by feeding. The minus (replicative) strand of SINV-3 was detected in worker ants indicating replication of the virus. The possibility of using SINV-3 as a microbial control agent for fire ants is discussed.
机译：我们报告从红色的进口火蚁Solenopsis invicta发现了一种新病毒。贪食贪腐菌3（SINV-3）代表使用宏基因组学方法从该蚂蚁物种中发现的第三种病毒。对SINV-3的单（正）链RNA，单部分双顺反子基因组进行了整体测序（GenBank登录号为FJ528584），由10,386个核苷酸组成，并在3'末端进行了聚腺苷酸化。通过Northern分析证实了该基因组大小。基因组在有义方向上揭示了2个大的开放阅读框（ORF），在每个末端以及两个ORF之间有一个非翻译区（UTR）。 5'近端ORF（ORF 1）编码的预测蛋白为299.1 kDa（2580个氨基酸）。 3'近端ORF（ORF 2）编码的预测蛋白为73.2 kDa（651个氨基酸）。在ORF 1中识别了RNA依赖性RNA聚合酶（RdRp），解旋酶和蛋白酶域。纯化SINV-3颗粒的SDS-PAGE分离产生2条带（表面为衣壳蛋白），总分子量为77.3 kDa，与ORF 2预测的质量（73.2 kDa）。从双顺反子病毒，ifla病毒，植物小RNA病毒，小核糖核酸病毒和4种未分配的正链RNA病毒对RdRp的结构域I到VIII的保守氨基酸序列进行系统进化分析，发现三叉形谱图与SINV-3和海带苍蝇病毒包含独特的集群。对SINV-3负染色样品的电子显微镜检查显示等轴测颗粒具有明显的突起，直径为27.3 +/- 1.3 nm。 SINV-3已通过进食成功地传播给未感染的工人。在工蚁中检测到SINV-3的负（复制）链，表明该病毒已复制。讨论了使用SINV-3作为火蚁的微生物控制剂的可能性。

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