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首页> 外文期刊>Chinese science bulletin >Influence of 5'-untranslated region (UTR) sequence on the regulation of human tissue plasminogen activator (t-PA) mRNA expression
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Influence of 5'-untranslated region (UTR) sequence on the regulation of human tissue plasminogen activator (t-PA) mRNA expression

机译：5'-非翻译区（UTR）序列对人类组织纤溶酶原激活物（t-PA）mRNA表达调控的影响

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摘要

Human tissue plasminogen activator (t-PA) is a serine protease that plays a key role in fibrinolysis, a process of dissolving blood clot. t-PA is produced by a variety of cells and tissues and many tumor cell lines. It is well known that t-PA expression in these cell lines is regulated at the transcriptional level. However, little is known about the translational regulation of t-PA expression. We have previously reported that the 3'-untranslated region (3'-UTR) may inhibit t-PA mRNA expression and identified a segment within 3'-UTR which is responsible for the translational regulation. The t-PA mRNA has a 5'-untranslated region (5'-UTR) of about 209 nucleotides. To investigate whether this sequence is involved in the regulation of t-PA expression, we have cloned 5' end sequence of t-PA cDNA from melanoma cells by reverse transcription and PCR, then studied the influence of 5'-UTR sequence on t-PA mRNA expression both in reticulocyte lysate and in COS-7 cells.
机译：人体组织纤溶酶原激活剂（t-PA）是一种丝氨酸蛋白酶，在纤维蛋白溶解（溶解血块的过程）中起关键作用。 t-PA由多种细胞和组织以及许多肿瘤细胞系产生。众所周知，这些细胞系中的t-PA表达在转录水平受到调控。然而，关于t-PA表达的翻译调控知之甚少。我们以前曾报道过3'-非翻译区（3'-UTR）可能抑制t-PA mRNA表达并鉴定了3'-UTR中负责翻译调控的区段。 t-PA mRNA具有约209个核苷酸的5'非翻译区（5'-UTR）。为了研究该序列是否参与t-PA表达的调控，我们通过逆转录和PCR从黑素瘤细胞克隆了t-PA cDNA的5'末端序列，然后研究了5'-UTR序列对t-PA表达的影响。网织红细胞裂解液和COS-7细胞中PA mRNA的表达。

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