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首页> 外文期刊>Water Science and Technology >Rapid detection of biofilms and adherent pathogens using scanning confocal laser microscopy and episcopic differential interference contrast microscopy
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Rapid detection of biofilms and adherent pathogens using scanning confocal laser microscopy and episcopic differential interference contrast microscopy

机译：使用扫描共聚焦激光显微镜和表观微分干涉对比显微镜快速检测生物膜和粘附的病原体

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Knowledge of biofilm structure and function has changed significantly in the last few years due to advances in light microscopy. One pertinent example is the use of scanning confocal laser microscopy (SCLM) to visualise corrosion pits caused by the biofilm mosaic footprint on corroding metal surfaces. Nevertheless, SCLM has some limitations as to its widespread use, including cost, inability to observe motile bacteria and eukaryotic grazers within biofilms, and difficulty to scan a curved surface. By contrast, episcopic differential interference contrast (EDIC) microscopy has provided a rapid, real time analysis of biofilms on opaque, curved, natural or man-made surfaces without the need for cover slips and oil. EDIC, coupled with epi-fluorescence (EDIC/EF), microscopy has been used successfully to visualise the 3-D biofilm structure, physiological niches, protozoal grazing and iron biomineralization, and the location of specific pathogens such as Legionella pneumophila, Campylobacter jejuni and Cryptosporidium parvum. These species were identified using gold nanoparticles or fluorophores coupled to monoclonal antibodies or 16S rRNA probes, respectively. Among its many potential uses, the EDIC technique will provide a rapid procedure to facilitate the calibration of the modern generation of biofilm-sensing electrodes. [References: 21]
机译：由于光学显微镜的进步，近年来生物膜结构和功能的知识已发生了巨大变化。一个相关的例子是使用扫描共聚焦激光显微镜（SCLM）可视化由腐蚀金属表面上的生物膜镶嵌足迹所引起的腐蚀坑。然而，SCLM在其广泛使用方面存在一些局限性，包括成本，无法观察生物膜内的活动细菌和真核放牧者以及难以扫描曲面。相比之下，镜检微分干涉对比（EDIC）显微镜可以快速，实时地分析不透明，弯曲，自然或人造表面上的生物膜，而无需盖玻片和油脂。 EDIC与落射荧光（EDIC / EF）结合使用，显微镜已成功地用于可视化3-D生物膜结构，生理生态位，原生动物放牧和铁生物矿化以及特定病原体（例如军团菌，空肠弯曲杆菌和小隐孢子虫。使用分别与单克隆抗体或16S rRNA探针偶联的金纳米颗粒或荧光团鉴定了这些物种。 EDIC技术在其许多潜在用途中，将提供一种快速的程序来促进对现代生物膜感应电极的校准。 [参考：21]

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