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首页> 外文期刊>Phytotherapy research: PTR >Modulating Bcl-2 family proteins and caspase-3 in induction of apoptosis by paeoniflorin in human cervical cancer cells
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Modulating Bcl-2 family proteins and caspase-3 in induction of apoptosis by paeoniflorin in human cervical cancer cells

机译：pa药苷诱导人宫颈癌细胞凋亡中调控Bcl-2家族蛋白和caspase-3的表达

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摘要

Paeoniflorin (PF), the principal bioactive component in the paeony root, has been used alone or combined with other herbs for many years in traditional Chinese medicine. New studies have shown that PF possesses an antitumor effect. However, the effect of PF on human cervical cancer cells has not been reported previously. This study determined the effect of PF on human cervical cancer cell line (HeLa) cells by the methyl thiazolyl tetrazolium (MTT) assay, flow cytometry with annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) technology, the transmission electron microscope (TEM) and immunocytochemical technique. After treatment with PF, the proliferation of HeLa cells was inhibited in a dose and time-dependent manner (p < 0.05). The apoptosis rate of HeLa cells increased with ascending concentrations of PF (p < 0.05) and the proportion of HeLa cells in S phase showed an increasing trend also. Typical apoptotic changes of HeLa cells exposed to PF were seen under the TEM. Meanwhile, there was a decrease in the expression of Bcl-2 and an enhancement in the expression of Bax and caspase-3 genes compared with the control group (p < 0.05). In conclusion, PF can induce significantly the apoptosis of HeLa cells, which may be demonstrated by the down-regulation of anti-apoptosis gene Bcl-2 and the up-regulation of pro-apoptosis genes Bax and caspase-3.
机译：eon药苷（PF）是pa药根中的主要生物活性成分，多年来在传统中药中已单独使用或与其他草药结合使用。新的研究表明，PF具有抗肿瘤作用。但是，PF对人宫颈癌细胞的作用以前没有报道。这项研究通过甲基噻唑基四唑鎓（MTT）测定，膜联蛋白V-异硫氰酸荧光素（FITC）/碘化丙啶（PI）技术，透射电子显微镜流式细胞术确定了PF对人宫颈癌细胞（HeLa）细胞的影响（TEM）和免疫细胞化学技术。 PF处理后，HeLa细胞的增殖受到剂量和时间依赖性的抑制（p <0.05）。 HeLa细胞的凋亡率随PF浓度的升高而增加（p <0.05），S期HeLa细胞的比例也呈上升趋势。在TEM下观察到暴露于PF的HeLa细胞典型的凋亡变化。同时，与对照组相比，Bcl-2的表达减少，而Bax和caspase-3的表达增加（p <0.05）。总之，PF可以显着诱导HeLa细胞凋亡，这可以通过抗凋亡基因Bcl-2的下调以及促凋亡基因Bax和caspase-3的上调来证明。

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