首页> 外文期刊>Weed Science: Journal of the Weed Science Society of America >The Evidence for Reduced Glyphosate Efficacy on Acetolactate Synthase-Inhibiting Herbicide-Resistant Yellow Nutsedge (Cyperus esculentus)
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The Evidence for Reduced Glyphosate Efficacy on Acetolactate Synthase-Inhibiting Herbicide-Resistant Yellow Nutsedge (Cyperus esculentus)

机译:草甘膦对乙酰乳酸合酶抑制除草剂抗性黄坚果(Cyperus esculentus)功效降低的证据

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A yellow nutsedge biotype (Res) from an Arkansas rice field has evolved resistance to acetolactate synthase (ALS)-inhibiting herbicides. The Res biotype previously exhibited cross-resistance to ALS inhibitors from four chemical families (imidazolinone, pyrimidinyl benzoate, sulfonylurea, and triazolopyrimidine). Experiments were conducted to evaluate alternative herbicides (i.e., glyphosate, bentazon, propanil, quinclorac, and 2,4-D) currently labeled in Arkansas rice-soybean production systems. Based on the percentage of aboveground dry weight reduction, control of the yellow nutsedge biotypes with the labeled rate of bentazon, propanil, quinclorac, and 2,4-D was 44%. Glyphosate (867 g ae ha(-1)) resulted in 68 and > 94% control of the Res and susceptible yellow nutsedge biotypes, respectively, at 28 d after treatment. Dose-response studies were conducted to estimate the efficacy of glyphosate on the Res biotype, three susceptible yellow nutsedge biotypes, and purple nutsedge. Based on the dry weights, the Res biotype was >= 5- and >= 1.3-fold less responsive to glyphosate compared to the susceptible biotypes and purple nutsedge, respectively. Differences in absorption and translocation of radiolabeled glyphosate were observed among the yellow nutsedge biotypes and purple nutsedge. The susceptible biotype had less C-14-glyphosate radioactivity in the tissues above the treated leaf and greater radioactivity in tissues below the treated leaf compared to the Res biotype and purple nutsedge. Reduced translocation of glyphosate in tissues below the treated leaf of the Res biotype could be a reason for the lower glyphosate efficacy in the Res biotype. No amino acid substitution that would correspond to glyphosate resistance was found in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene of the Res biotype. However, an amino acid (serine) addition was detected in the EPSPS gene of the Res biotype; albeit, it is not believed that this addition contributes to lower efficacy of glyphosate in this biotype.
机译:一种来自阿肯色州稻田的黄色坚果生物型(Res)已进化出对抑制乙酰乳酸合酶(ALS)的除草剂的抗性。 Res生物型以前对来自四个化学家族(咪唑啉酮,嘧啶基苯甲酸酯,磺酰脲和三唑并嘧啶)的ALS抑制剂表现出交叉耐药性。进行了实验以评估目前在阿肯色州大米-大豆生产系统中标记的替代除草剂(即草甘膦,苯达松,丙烷,喹克洛拉和2,4-D)。基于地上干重减少的百分比,以标记的苯达松,丙烷,喹克洛拉和2,4-D标记的黄色无核生物型的控制率为<44%。草甘膦(867 g ae ha(-1))在处理后28 d分别导致Res和易感黄色甜菜型生物型的控制为68%和> 94%。进行了剂量反应研究,以评估草甘膦对Res生物型,三种易感的黄色甜菜型和紫色甜菜的功效。基于干重,Res生物型对草甘膦的响应分别比易感生物型和紫色坚果少> = 5和> = 1.3倍。在黄色营养型和紫色营养型之间观察到了放射性标记草甘膦的吸收和转运差异。与Res生物型和紫色小坚果相比,易感生物型在处理过的叶子上方的组织中具有较少的C-14-草甘膦放射性,而在处理过的叶子下方的组织中具有较高的放射性。草甘膦在Res生物型的经处理的叶子下方的组织中减少的易位性可能是Res生物型中草甘膦功效较低的原因。在Res生物型的5-烯丙基丙酮酸shi草酸酯-3-磷酸合酶(EPSPS)基因中未发现对应于草甘膦抗性的氨基酸取代。但是,在Res生物型的EPSPS基因中检测到了氨基酸(丝氨酸)的添加。尽管不相信这种添加会降低该生物型中草甘膦的功效。

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