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首页> 外文期刊>The Journal of Eukaryotic Microbiology >Next-Generation Sequencing Assessment of Eukaryotic Diversity in Oil Sands Tailings Ponds Sediments and Surface Water
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Next-Generation Sequencing Assessment of Eukaryotic Diversity in Oil Sands Tailings Ponds Sediments and Surface Water

机译：油砂尾矿池沉积物和地表水中真核生物多样性的下一代测序评估

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Tailings ponds in the Athabasca oil sands (Canada) contain fluid wastes, generated by the extraction of bitumen from oil sands ores. Although the autochthonous prokaryotic communities have been relatively well characterized, almost nothing is known about microbial eukaryotes living in the anoxic soft sediments of tailings ponds or in the thin oxic layer of water that covers them. We carried out the first next-generation sequencing study of microbial eukaryotic diversity in oil sands tailings ponds. In metagenomes prepared from tailings sediment and surface water, we detected very low numbers of sequences encoding eukaryotic small subunit ribosomal RNA representing seven major taxonomic groups of protists. We also produced and analysed three amplicon-based 18S rRNA libraries prepared from sediment samples. These revealed a more diverse set of taxa, 169 different OTUs encompassing up to eleven higher order groups of eukaryotes, according to detailed classification using homology searching and phylogenetic methods. The 10 most abundant OTUs accounted for >90% of the total of reads, vs. large numbers of rare OTUs (1% abundance). Despite the anoxic and hydrocarbon-enriched nature of the environment, the tailings ponds harbour complex communities of microbial eukaryotes indicating that these organisms should be taken into account when studying the microbiology of the oil sands.
机译：阿萨巴斯卡油砂（加拿大）的尾矿池中含有废液，这些废液是从油砂矿石中提取沥青产生的。尽管自生的原核生物群落具有相对较好的特征，但对生活在尾矿池缺氧软沉积物中或覆盖它们的薄水层中的微生物真核生物几乎一无所知。我们对油砂尾矿池中的微生物真核生物多样性进行了首次下一代测序研究。在由尾矿沉积物和地表水制备的元基因组中，我们检测到极少量的编码代表七个主要生物分类群的真核小亚基核糖体RNA的序列。我们还生产并分析了三个由沉积物样品制备的基于扩增子的18S rRNA文库。根据使用同源搜索和系统发育方法的详细分类，这些揭示了一组更多样化的分类单元，包括169个不同的OTU，包括多达11个高阶真核生物。 10个最丰富的OTU占读取总数的90％以上，而大量的稀有OTU（丰度<1％）。尽管环境具有缺氧和富含碳氢化合物的性质，但尾矿池中还是微生物真核生物的复杂群落，这表明在研究油砂微生物时应考虑这些生物。

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