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RAPD marker linked with sbm-1, a gene conferring resistance to pea seed-born mosaic virus

机译:RAPD标记与sbm-1连锁,该基因赋予对豌豆种子天生的花叶病毒的抗性

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摘要

DNA markers linked with trait of disease-resistance offer a very useful tool for selection of putative disease-resistant progeny and cloning disease-resistant genes. Pea seed-born mosaic virus (PSbMV) is spread mainly through planting infected pea seeds and by non-persistent aphid transmission. Three strains of PSbMV have been identified, designated as P-1, L and P-4. Strain P-1 is most widely distributed. Four recessive genes conferring resistance to the three known strains have been identified in previous studies: sbm-1 against P-1, sbm-2 and sbm-3 against both L and the related L-1 variant and sbm-4 against P-4. In 1993, Timmerman first reported the restriction fragment length polymorphism (RFLP) marker linked with sbm-1 separated by a distance of 8 cM. In this study, the authors identified a random amplified polymorphic DNA (RAPD) marker closely linked with sbm-1 based on bulked segregant analysis (BSA) method.
机译:与抗病性相关的DNA标记为选择推定的抗病后代和克隆抗病基因提供了非常有用的工具。豌豆种子出生的花叶病毒(PSbMV)主要通过种植受感染的豌豆种子和非持久性蚜虫传播而传播。已经鉴定出三种PSbMV菌株,分别命名为P-1,L和P-4。 P-1菌株分布最广。在先前的研究中已鉴定出四个对三个已知菌株具有抗性的隐性基因:针对P-1的sbm-1,针对L和相关L-1变体的sbm-2和sbm-3以及针对P-4的sbm-4 。 1993年,Timmerman首次报道了与sbm-1相关的限制性片段长度多态性(RFLP)标记,其间隔为8 cM。在这项研究中,作者基于大量分离物分析(BSA)方法鉴定了与sbm-1紧密相关的随机扩增多态性DNA(RAPD)标记。

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