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Biochemical analysis of argyrophilic nonhistone proteins in chromosomes of plant Triticum aestivum

机译:植物小麦染色体中嗜银非组蛋白的生化分析。

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IN the late 1970s, biochemical analysis and electron microscopy of isolated, histone-depleted mitotic chromosomes demonstrated the existence of a central scaffold consisting mainly of nonhistone proteins (NHPs) in metaphase chromosomes. Subsequently,by using silver impregnation techniques and light microscopy, Howell et al. showed the presence of an axial chromatid core in relatively intact chromosomes of mammals; this core is believed to be similar to the scaffold seen in histone-depleted chromosomes. Cytochemical studies have indicated that the core components responsible for silver staining are largely chromosomal NHPs. Earn-shaw et al. further showed that the silver-stained core observed in relatively intact chromosomes is related to the scaffold present in histone-depleted chromosomes, and suggested that the nonhistone scaffold is the principal target of the silver stain.
机译:在1970年代后期,离体的,组蛋白耗尽的有丝分裂染色体的生化分析和电子显微镜观察表明,在中期染色体中存在主要由非组蛋白(NHP)组成的中央支架。随后,通过使用银浸渍技术和光学显微镜,Howell等人。表明在哺乳动物相对完整的染色体中存在轴向染色单体核心;据信该核心类似于在组蛋白耗尽的染色体中看到的支架。细胞化学研究表明,负责银染的核心成分主要是染色体NHP。 Earn-shaw等。进一步表明,在相对完整的染色体中观察到的银染核心与存在于组蛋白耗尽的染色体中的支架有关,并表明非组蛋白支架是银染的主要目标。

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