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Synthesis and characterization of model conjugate of phycobilisomes

机译：藻胆体模型偶联物的合成与表征

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摘要

PHYCOBILISOMES are light-harvesting protein-pigment complexes located on the cytoplasmic side of the thylakoid membranes in cyanobacteria and red algae. They absorb visible light in the 550--660 nm range of the spectrum and transfer the energy to chlorophyll a. The main components of the phycobilisomes are phycoerythrin (PE), phycocyanin (PC) and allophyco-cyanin (APC). Phycobilisomes consist of APC cores surrounded by PC and PE on the periphery. APC functions as the bridging pigment between phycobilisomes and the photosynthetic lamellae. The energy transfer direction concluded from the results of steady-state spectra is from PE to PC and finally to APC. Furthermore, quantitative information can be obtained from the time-resolved fluorescence and absorption spectra. However, because of the complexity of the structure of the separated phycobilisomes, the energy transfer kinetics in PBS is not clear up to now. For this reason, the phycobilisome model conjugate in which the phyco-biliproteins are linked by chemical bonds was synthesized. Furthermore, phycobilipfoteins give high fluorescence quantum yields and possess the property of energy transfer. Therefore, the tandem phycobiliprotein conjugates can be used in fluorescence-activated cell sorting,fluorescence microscopy, and fluorescence immunoassay analysis.
机译：PHYCOBILISOMES是位于蓝藻和红藻类囊体膜细胞质侧的光捕获蛋白-色素复合物。它们吸收光谱在550--660 nm范围内的可见光，并将能量转移到叶绿素a。藻胆体的主要成分是藻红蛋白（PE），藻蓝蛋白（PC）和别藻蓝蛋白（APC）。藻胆小体由被PC和PE围绕的APC核心组成。 APC充当藻胆小体和光合薄片之间的桥梁色素。从稳态光谱结果得出的能量转移方向是从PE到PC，最后到APC。此外，可以从时间分辨的荧光和吸收光谱获得定量信息。然而，由于分离的藻胆体结构的复杂性，目前尚不清楚PBS中的能量转移动力学。因此，合成了通过化学键连接藻胆蛋白的藻胆体模型结合物。此外，藻胆粉蛋白具有高的荧光量子产率并具有能量转移的特性。因此，串联藻胆蛋白缀合物可用于荧光激活细胞分选，荧光显微镜和荧光免疫分析。

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