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首页> 外文期刊>Chinese science bulletin >Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets (Cl) mutant
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Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets (Cl) mutant

机译：水稻簇生小穗（Cl）突变体的形态学和作图分析

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摘要

The rice clustered spikelets (Cl) mutant exhibits a phenotype that most of branch apical have 2-3 spikelets clustered together. SEM (scanning electron microscope) observation suggested that the Cl gene controlled branch apical development, and influenced the terminal spikelets elongation. The spikelet number was reduced in mutant, indicating that Cl may also have an effect on spikelet number. To map Cl locus, two F_2 mapping populations derived from the crosses between the Cl and ZhongHua11, and Cl and ZheFu802 were constructed, respectively. The Cl locus was roughly mapped between two CAPS markers, CK0214 and SS0324. A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl2560, with genetic distances of 0.2 and 2.1 cM, respectively. Then we found a PAC contig spanning Cl locus, the region was delimited to 196 kb. This result was useful for cloning of the Cl gene. Allelism test demonstrated that Cl was allelic to Cl2, another rice clustered spikelets mutant.
机译：水稻簇生的小穗（Cl）突变体表现出大多数分支顶端具有2-3个聚集在一起的小穗的表型。扫描电镜（SEM）的观察表明，Cl基因控制着分支的顶端发育，并影响了末端小穗的伸长。突变体中小穗数减少，表明C1也可能对小穗数有影响。为了绘制Cl位点，分别构建了两个Cl和ZhongHua11之间的杂交得到的F_2作图种群。 Cl位点被粗略地映射在两个CAPS标记CK0214和SS0324之间。进一步的精细作图分析表明，C1基因座位于制图人R0674E和Cl2560之间，遗传距离分别为0.2和2.1 cM。然后我们发现了一个跨越C1基因座的PAC重叠群，该区域被限定为196kb。该结果对于克隆C1基因是有用的。等位测试表明，Cl与另一个水稻簇状小穗突变体Cl2等位。

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