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首页> 外文期刊>World Journal of Microbiology & Biotechnology >Intergeneric protoplast fusion between Calocybe indica (milky mushroom) and Pleurotus florida aids in the qualitative and quantitative improvement of sporophore of the milky mushroom
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Intergeneric protoplast fusion between Calocybe indica (milky mushroom) and Pleurotus florida aids in the qualitative and quantitative improvement of sporophore of the milky mushroom

机译：乳白蘑菇（Calocybe indica）和佛罗里达平菇（Pleurotus florida）之间的原生质体融合有助于定性和定量改善乳蘑菇的孢子体

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This article primarily focuses on the production of somatic hybrid sporophores through PEG-mediated protoplast fusion between Calocybe indica var. APK2 and Pleurotus florida. To screen the hybrid strains a selection strategy was followed based on the differential tolerance of NaCl level by the two parental genera. Basidiocarps could be successfully generated from eight out of fourteen hybrid lines that were maintained in culture. Hybridity of the fusant lines was established on the basis of their colony morphology, mycelial growth rate and hyphal traits, while the fruit-body-generating lines were demarcated on the basis of nature of sporophores, isozyme and RAPD markers. The degree to which the hybrid population differed among themselves and from their parents was assessed by analysing each of these morphological variables by one-way analysis of variance (ANOVA). RAPD bands of the fusant lines were similar to either parental bands or were new non-parental bands, which classified them into the microgenome and macrogenome insertion types. A dendrogram created with the help of UPGMA method of clustering and Euclidean distance exhibited three major clusters, in which the Pleurotus-Calocybe hybrids showed intra-cluster variations. Notably, P. florida was genetically distant from the hybrid lines, while C. indica was phylogenetically the dominant parent. Significant increase in bio-efficiency and d-linoleic acid content in these hybrid lines indicated quantitative as well as qualitative improvement of the newly developed somatic hybrids.
机译：本文主要侧重于通过Cal介孢Cal之间的PEG介导的原生质体融合生产体细胞杂种孢子体。 APK2和佛罗里达侧耳。为了筛选杂交菌株，基于两个亲本属的NaCl水平的不同耐受性，遵循选择策略。从培养的十四个杂种系中的八个杂种成功地产生了巴西棕榈果。根据菌落的形态，菌丝生长速度和菌丝性状建立融合系的杂交性，并根据孢子体，同工酶和RAPD标记物的性质划分出产果体系。通过单向方差分析（ANOVA）分析这些形态变量，可以评估杂种种群之间及其与父母之间的差异程度。融合系的RAPD谱带与亲本谱带相似，或者是新的非亲本谱带，将它们分为微型基因组和宏基因组插入类型。借助UPGMA聚类方法和欧几里得距离生成的树状图显示出三个主要簇，其中侧耳属-Calocybe杂种表现出簇内变异。值得注意的是，佛罗里达假单胞菌在遗传上与杂种系相距遥远，而印度假单胞菌在系统发育上是优势亲本。这些杂交品系中生物效率和d-亚油酸含量的显着提高表明新近开发的体细胞杂种在数量上和质量上都有提高。

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