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首页> 外文期刊>World Journal of Microbiology & Biotechnology >Effect of oxidizing and non-oxidizing biocides on biofilm at different substrate levels in the model recirculating cooling water system.
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Effect of oxidizing and non-oxidizing biocides on biofilm at different substrate levels in the model recirculating cooling water system.

机译：在循环冷却水系统模型中，氧化和非氧化杀菌剂对不同底物水平生物膜的影响。

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摘要

With the reducing of water resources, using advanced treated refinery wastewater as recirculating cooling water is an effective method to save water and to reduce the pollution of petroleum and petrochemical industry. However, the control of biofilm is a bottleneck in the application of this technology. To resolve the problem of biofilm formation and development, antimicrobial characteristics of chlorine dioxide and benzyldimethyldodecyl-ammonium chloride on biofilm at different substrate levels were investigated. Biofilm detachment ratio and TTC-dehydrogenase activity (DHA) were two indexes to discuss the antimicrobial effects. The results showed that at the high substrate level, the biofilms characteristics (biomass, the content of protein, polysaccharide and EPS) were the higher than those at the medium and low substrate levels, however biofilm's DHA at the medium substrate level (12.97 micro gTF/(g h)) was higher than those at the medium substrate level (7.64 micro gTF/(g h)) and low substrate level (1.94 micro gTF/(g h)). The difference of substrate level in the media resulted in different biofilm structure. By contrast with the control experiment, biofilm detachment ratios were all increased in three media with ClO2 and BDMDAC addition. After ClO2 addition, MITs were 30, 120 and 240 min and MIC was 1, 4 and 6 mg/L, respectively, at the low, medium and high substrate level. After BDMDAC addition, MITs in three media were all longer than those after ClO2 addition, MIC was 200, 300 and 400 mg/L, respectively, at the low, medium and high substrate level.
机译：随着水资源的减少，使用经过处理的精炼废水作为循环冷却水是节约用水，减少石油和石化工业污染的有效方法。但是，生物膜的控制是该技术应用的瓶颈。为了解决生物膜形成和发展的问题，研究了在不同底物水平下二氧化氯和苄基二甲基十二烷基氯化铵在生物膜上的抗菌特性。生物膜分离率和TTC-脱氢酶活性（DHA）是讨论抗菌作用的两个指标。结果表明，在高底物水平下，生物膜特性（生物量，蛋白质，多糖和EPS含量）高于中低底物水平，而在中底物水平（12.97 micro gTF）下生物膜的DHA较高。 /（gh））高于中底物水平（7.64 micro gTF /（gh））和低底物水平（1.94 micro gTF /（gh））的水平。培养基中底物水平的差异导致不同的生物膜结构。与对照实验相比，在添加ClO 2 和BDMDAC的三种培养基中，生物膜的脱落率均增加。加入ClO 2 后，在低，中和高底物水平下，MIT分别为30、120和240分钟，MIC为1、4和6 mg / L。加入BDMDAC后，三种培养基中的MIT均比加入ClO 2 后的MIT长，在低，中和高底物水平下MIC分别为200、300和400 mg / L。

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