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首页> 外文期刊>Chinese science bulletin >Fine mapping of a semidwarf gene sd-g in indica rice (Oryza sativa L.)
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Fine mapping of a semidwarf gene sd-g in indica rice (Oryza sativa L.)

机译:war稻(Oryza sativa L.)中半矮基因sd-g的精细定位

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摘要

The semidwarf gene sd-g which has been used in indica rice breeding in southern China is a new one, nonallelic to sd-l. To map sd-g, an F_2 population derived from the cross between xinguiaishuangai and 02428 was constructed. The sd-g was roughly mapped between two mirosatellite markers RM440 and RM163, with genetic distances of 0.5 and 2.5 cM, respectively. Then nine new polymorphic microsatellite markers were developed in this region. The sd-g was further mapped between two microsatellite markers SSR5-1 and SSR5-51, with genetic distances of 0.1 and 0.3 cM, respectively, while cosegregated with SSR418. A BCA contig was found to span the sd-g locus, the region being delimited to 85 kb. This result was very useful for cloning of the sd-g gene.
机译:在中国南方的in稻育种中使用的半矮基因sd-g是一种新的,与sd-1不等位的基因。为了绘制sd-g图谱,构建了一个来自兴uiaishuangai和02428之间杂交的F_2种群。 sd-g粗略地定位在两个微卫星标记RM440和RM163之间,遗传距离分别为0.5和2.5 cM。然后在该区域开发了九个新的多态微卫星标记。将sd-g进一步定位在两个微卫星标记SSR5-1和SSR5-51之间,遗传距离分别为0.1和0.3 cM,同时与SSR418共分离。发现BCA重叠群跨越sd-g基因座,该区域限定为85kb。该结果对于sd-g基因的克隆非常有用。

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