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首页> 外文期刊>Chinese science bulletin >Cytotoxicity of HIV-gp~(41) segments expressed in E. coli
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Cytotoxicity of HIV-gp~(41) segments expressed in E. coli

机译：在大肠杆菌中表达的HIV-gp〜（41）片段的细胞毒性

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摘要

The failed attempt to express HIV- gp~(41) in E. coli led to the investigation of HIV- gp~(41) segments, which is responsible for the toxicity to E. coli cells. A series of deletion mutants containing different regions of gp~(41) gene were constructed and expressed in E. coli BL21(DE3) strain. After IPTG induction, the high mortality of host bacteria was observed in host bacteria carrying the deletion mutants of gp~(41) gene except for those transformed with pET-HN2; coordinately, the mRNA transcripts of the gp~(41) was rapidly decreased; and the release of [~3H]uridine increased upon induction. All these data suggested that GP41 protein has a cytotoxic effect on E. coli, and it is the cytotoxicity of the gp~(41) gene product that contributes to the high mortality when expressed in E. coli.
机译：未能在大肠杆菌中表达HIV- gp〜（41）的尝试失败，导致了对HIV-gp〜（41）片段的研究，这是对大肠杆菌细胞毒性的原因。构建了一系列包含gp〜（41）基因不同区域的缺失突变体，并在大肠杆菌BL21（DE3）菌株中表达。 IPTG诱导后，在携带gp〜（41）基因缺失突变体的宿主细菌中观察到宿主细菌的高死亡率，除了用pET-HN2转化的那些。协调地，gp〜（41）的mRNA转录物迅速减少。诱导后[〜3H]尿苷的释放增加。所有这些数据表明，GP41蛋白对大肠杆菌具有细胞毒性作用，而gp〜（41）基因产物的细胞毒性在大肠杆菌中表达时导致高死亡率。

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