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首页> 外文期刊>World Journal of Microbiology & Biotechnology >Drosera peltata Smith var. lunata (Buch.-Ham.) C. B. Clarke as a feasible source of plumbagin: phytochemical analysis and antifungal activity assay
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Drosera peltata Smith var. lunata (Buch.-Ham.) C. B. Clarke as a feasible source of plumbagin: phytochemical analysis and antifungal activity assay

机译：Drosera peltata Smith变种lunata（Buch.-Ham。）C. B. Clarke作为铅白蛋白的可行来源：植物化学分析和抗真菌活性测定

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摘要

Drosera peltata Smith var. lunata (Buch.-Ham.) C. B. Clarke (DPVL) fractions and plumbagin were tested via broth microdilution techniques on Rhizopus oryzae, Aspergillus flavus, Aspergillus niger, Aspergillus oryzae, Penicillium citrinum. All of the test substances [petroleum ether, chloroform, ethyl acetate, n-butanol fraction and aqueous residue (AR)] except for the AR were active against all the tested strains. The petroleum ether fraction (PEF) was the most active (MIC = 5.86-46.88 mu g/ml, MFC = 23.44-93.75 mu g/ml) of the five tested substances and therefore, was selected for further analysis. Based on antifungal activity, bioactivity-guided fractionation of the PEF led to the isolation of plumbagin. The structure of plumbagin was elucidated by H-1 and C-13 NMR. Using HPLC, DPVL was found to be a new source of plumbagin. Reversed-phase HPLC was performed using a mobile phase of water and methanol, and peaks were detected at 254 nm. Plumbagin showed a good linear relationship at concentrations ranging from 0.625 to 10 mu g/ml. Both the intraday and the interday precision showed that the method was precise, with RSDs of at least 3 % at different concentrations. Recovery rates ranging from 97.86 to 99.94 % were observed, which indicate that the method is accurate. The specificity of the method was established by checking the peak purity of plumbagin. For six independent measurements, the average plumbagin content in DPVL was 11.05 +/- A 0.31 mg/g of dried material. The validated HPLC method provides a new basis for assessing DPVL quality.
机译：Drosera peltata Smith变种通过肉汤微量稀释技术在米根霉，黄曲霉，黑曲霉，米曲霉，柠檬青霉上通过肉汤微稀释技术测试月桂（Buch.-Ham。）C.B。克拉克（DPVL）级分和李白蛋白。除AR外，所有测试物质[石油醚，氯仿，乙酸乙酯，正丁醇馏分和含水残留物（AR）]对所有测试菌株均具有活性。在五种被测物质中，石油醚馏分（PEF）最具活性（MIC = 5.86-46.88μg / ml，MFC = 23.44-93.75μg/ ml），因此被选择用于进一步分析。基于抗真菌活性，PEF的生物活性指导分级分离导致了铅青霉素的分离。通过H-1和C-13 NMR阐明了李白蛋白的结构。使用HPLC，发现DPVL是羽扇豆苷的新来源。使用水和甲醇的流动相进行反相HPLC，并在254 nm处检测到峰。铅白蛋白在0.625至10μg / ml的浓度范围内表现出良好的线性关系。日内和日间精度均表明该方法是精确的，不同浓度下的RSD至少为3％。回收率在97.86到99.94％之间，表明该方法是准确的。该方法的特异性是通过检查plumbagin的峰纯度确定的。对于六次独立测量，DPVL中的平均铅皮蛋黄素含量为11.05 +/- 0.31 mg / g干物质。经过验证的HPLC方法为评估DPVL质量提供了新的依据。

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