首页> 外文期刊>World Journal of Microbiology & Biotechnology >Fungicide resistance of Botrytis cinerea in tomato greenhouses in the Canary Islands and effectiveness of non-chemical treatments against gray mold
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Fungicide resistance of Botrytis cinerea in tomato greenhouses in the Canary Islands and effectiveness of non-chemical treatments against gray mold

机译:加那利群岛番茄温室番茄灰霉病菌的抗药性及非化学处理对灰霉病的有效性

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Tomato greenhouses in the Canary Islands, Spain, were surveyed to estimate frequencies of resistance to benzimidazoles, dicarboximides, anilinopyrimidines and N-phenylcarbamates in Botrytis cinerea. Resistance to carbendazim, iprodione, pyrimethanil and diethofencarb was found in 74.2, 86.4, 28.8 and 31.8 % of isolates, respectively. Benzimidazole-and anilinopyrimide-resistant isolates were highly resistant, showing EC50 values above 500 mu g/ml carbendazim and a mean EC50 value of 28.42 mu g/ml pyrimethanil, respectively. By contrast, a low level of resistance was observed among dicarboximide-resistant isolates (mean EC50 value of 1.81 mu g/ml iprodione). Phenotypes with double resistance to carbendazim and iprodione, and triple resistance to carbendazim, iprodione and pyrimethanil were the most common, occurring in 36.4 and 28.8 % of isolates. The surveyed greenhouses had never been treated with fenhexamid and Signum (TM) (pre-packed mixture of boscalid and pyraclostrobin), and baseline sensitivities of B. cinerea isolates to these fungicides were determined. The EC50 values were within the range of 0.009-0.795 mu g/ml fenhexamid and of 0.014-0.48 mu g/ml Signum. In addition, available formulations based on elicitors of plant defense response and biocontrol agents were evaluated against B. cinerea in tomato plants under semi-controlled greenhouse conditions, the yeast Candida sake CPA-1 being able to reduce gray mold significantly when it was applied on petiole wounds and the plants were inoculated 24 h later. Likewise, C. sake was effective against B. cinerea in harvested tomato fruits, yeast-treated tomatoes showed a 70.66 and 30.31 % reduction in the diameters of decay lesions compared with controls after 10 days of storage at 20 and 9 degrees C, respectively
机译:对西班牙加那利群岛的番茄温室进行了调查,以估计灰葡萄孢中对苯并咪唑,二甲酰亚胺,苯胺嘧啶和N-苯基氨基甲酸酯的抗性频率。在分离株中分别发现对多菌灵,异丙隆,嘧菌腈和二氢呋喃卡宾具有抗性,分别为74.2%,86.4%,28.8%和31.8%。耐苯并咪唑和苯胺嘧啶的分离物具有高度抗性,分别显示多菌灵500μg/ ml多菌灵的EC50值和嘧菌腈28.42μg/ ml的平均EC50值。相反,在耐二甲羧酰亚胺的菌株中观察到了低水平的耐药性(平均EC50值为1.81μg/ ml异丙洛酮)。对多菌灵和异丙隆具有双抗性,对多菌灵,异丙洛酮和嘧啶醇具有三重抗性的表型是最常见的,在分离株中占36.4%和28.8%。被调查的温室从未使用过苯六胺和Signum™(预先包装的鳞状鳞茎和吡菌胺酯的混合物)处理过,并确定了灰葡萄双歧杆菌分离株对这些杀真菌剂的基线敏感性。 EC50值在0.009-0.795μg/ ml苯六胺和0.014-0.48μg/ ml Signum的范围内。此外,在半控制温室条件下,评估了基于植物防御反应引发剂和生物防治剂的可用制剂对番茄中的B. cinerea的抗灰霉病性能。酵母假丝酵母CPA-1能够显着减少灰霉病。叶柄伤口和植物接种后24小时。同样,清酒梭菌对收获的番茄果实中的灰葡萄孢也有效,经酵母处理的番茄分别在20和9摄氏度下存放10天后,与对照组相比,腐烂病的直径降低了70.66%和30.31%

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