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Regeneration of fertile plants from isolated tobacco zygotes by in vitro culture

机译：通过离体培养从分离的烟草合子再生可育植物

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摘要

Living zygotes of tobacco (Nicotiana Tabacum L.) SR-1 were isolated and cultured in vitro by the microculture technique. Fertile plants were regenerated from the calli derived from cultured zygotes via organogenesis. Ovules were collected 120 h after pollination and used as feeder. MS combined with KM8p was selected as basic medium in the experiment. Zzygotes isolated from ovules 108 h after pollination turned out to be suitable material for in vitro culture. Over 80 percent such zygotes could divide and around 10 percent of them could grow into calli and regenerate fertile plants.
机译：分离出烟草（Nicotiana Tabacum L.）SR-1的活合子，并通过微培养技术进行了体外培养。通过器官发生从培养的受精卵衍生的愈伤组织中再生出可育植物。授粉120小时后收集胚珠，用作饲养线。实验中选择了结合KM8p的MS作为基本培养基。授粉后108 h从胚珠分离的合子被证明是适合体外培养的材料。超过80％的这种受精卵可以分裂，其中约10％可以长成愈伤组织并再生可育植物。

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