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Evidence for hydrogen-deuterium exchange in viral particles

机译：病毒颗粒中氢-氘交换的证据

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摘要

Heavy water (D_2O) could enhance thermosta-bility of some viruses. However, the underlying mechanism is not clear. Here we report the development of a matrix-aided gas-isotope-ratio mass spectrometry method that allows direct determination of deuterium/hydrogen (D/H) ratio in D_2O-treated Japanese encephalitis virus (JEV), hepatitis A virus (HAY) and RNA from D_2O-treated HAV. The D/H ratio was expressed as delta D_(SMOW). Our experiments showed that delta D_(SMOW)values increased significantly in D_2O-treated viral samples compared to normal controls, and increment in delta D_(SMOW) of D_2O, treated viral samples was in a fine linear relationship with increment in amount of samples loaded in BSA matrix. Our experiments also indicated that increaseddelta D_(SMOW) of D_2O-treated virus correlated well with its enhanced thermostability. The results suggested that hydrogen-deuterium exchange occurred in viral particles and its RNA structure as a result of D_2O-treatment. Furthermore, such exchange could cause changes in viral phcnotype, such as enhanced thermostability.
机译：重水（D_2O）可以增强某些病毒的热稳定性。但是，其潜在机制尚不清楚。在这里，我们报告了基质辅助气体同位素比质谱方法的发展，该方法可以直接测定D_2O治疗的日本脑炎病毒（JEV），甲型肝炎病毒（HAY）和乙型肝炎病毒中的氘/氢（D / H）比。 D_2O处理的HAV中的RNA。 D / H比表示为ΔD_（SMOW）。我们的实验表明，与正常对照相比，D_2O处理的病毒样品中的D_（SMOW）值显着增加，D_2O处理的病毒样品中的D_（SMOW）的增量D_（SMOW）的增加与样品加载量的增加呈良好的线性关系在BSA矩阵中。我们的实验还表明，经D_2O处理的病毒的增量D_（SMOW）与其增强的热稳定性密切相关。结果表明，D_2O处理可导致病毒颗粒及其RNA结构发生氢-氘交换。此外，这种交换可以引起病毒表型的改变，例如增强的热稳定性。

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