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首页> 外文期刊>Chinese science bulletin >Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf
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Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf

机译：济南红十字会黄牛的克隆及其克隆牛的生殖特性评估

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Somatic cell done technology is a viable approach to preserving endangered livestock and wildlife genetic resources. la the present research, somatic cell nuclear transfer (SCNT) was performed using granulose cells from the critical endangered Chinese red-cross yellow cattle as donor cells. A total of 211 ocytes were manipulated and 166 v) of them were successfully enucleated. 112 (67.4 percent) embryos were reconstructed, 94 (83 percent) of them cleaved, and 48 (43 percent) of them developed to blastocyst stage. SCNT blastocysts were transferred to 6 Holstein recipients, and 2 (33 percent) of them were found to be pregnant. One of them maintained to term and delivered a calf, whereas another aborted. Effect of different fusion buffer (mannitol vs. Zimmerman fusion buffer) and different. activation methods (calcium ionophore+6-DMAP vs. cydoheximide +CB) on fusion rate and development of SCNT embryos were investigated. The results indicated that: (i) on condition of two DC pulses of 2.5 kV/cm for 18 fis each, fusion rates were higher in mannitol solution than in Zimmerman fusion buffer (71 percent vs. 61 percent, respectively, < 0.05), but the blastocysts rates did not differ between two treatments (36 percent vs. 39 percent, p>0.05 ); (ii) There was no significant difference in development rates to the blastocyst stage for SCNT embryos activated by calcium ionophore+6-DMAP or by cycloheximideCB (42 percent vs. 46 percent, respectively, p>0.05). Microsatellite DNA analysis examining 28 loci confirmed that the cloned calf was genetically identical to the donor Jinan red-cross yellow cattle and different from the recipient females. Growth and reproductive performance of cloned cow were evaluated, and there were no difference i cross-red n it between cloned and normal control Jinan yellow cattle. Furthermore, the cloned yellow cow has delivered a healthy yellow calf.
机译：体细胞完成技术是保存濒临灭绝的牲畜和野生动植物遗传资源的可行方法。在本研究中，体细胞核移植（SCNT）是使用来自濒危的中国红十字会黄牛的颗粒细胞作为供体细胞进行的。总共对211个细胞进行了操作，其中166 v）被成功摘除。重建了112个胚胎（占67.4％），其中有94个胚胎（占83％）被切割，其中48个胚胎（占43％）发育到了胚泡期。将SCNT囊胚转移到6个荷斯坦受体中，其中2个（33％）被发现怀孕。他们中的一个维持了足月并交付了一个小牛，而另一个则流产了。不同融合缓冲液（甘露醇与齐默曼融合缓冲液）的效果不同。研究了活化方法（钙离子载体+ 6-DMAP vs. cydoheximide + CB）对融合速率和SCNT胚胎发育的影响。结果表明：（i）在两个直流脉冲分别为2.5 kV / cm，持续18 fis的条件下，甘露醇溶液的融合率高于Zimmerman融合缓冲液（分别为71％和61％，<0.05），但是两种治疗的囊胚率没有差异（36％vs. 39％，p> 0.05）; （ii）被钙离子载体+ 6-DMAP或被环己酰亚胺CB激活的SCNT胚胎到胚泡期的发育速率没有显着差异（分别为42％和46％，p> 0.05）。对28个基因座进行的微卫星DNA分析证实，克隆的小牛在遗传上与供体济南红十字黄牛相同，并且与受体雌性不同。评价了克隆奶牛的生长和生殖性能，在克隆和正常对照济南黄牛之间没有交叉红色差异。此外，克隆的黄牛已经交付了健康的黄牛犊。

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