Phytophthora elicitor PB90 induced apoptosis in suspension cultures of tobacco

JI Rui; ZHANG Zhengguang; WANG Yuanchao

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Phytophthora elicitor PB90 induced apoptosis in suspension cultures of tobacco

机译：疫霉PB90诱导烟草悬浮培养细胞凋亡。

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The protein elicitor PB90 secreted by Phy-tophthora boehmeriae is an efficient elicitor inducing the hypersensitive response and systemic acquired resistance in tobacco plants. Here, we observed cell death in suspension-cultured cells of Nicotiana tobacum BY-2 with PB90 treatment using Trypan blue staining method. And this cell death could be suppressed by cydoheximide, an inhibitor of proteins synthesis, which implies that PB90-induced cell death was an active cell death process requiring new protein synthesis. DAPI staining revealed that PB90 induce rapid chromatin condensation, margination, apoptotic bodies' formation and DMA laddering, further TUNEL assay also observed the specific breakage of 3'-OH ends. All of the above common morphological characteristics indicated that PB90 induced apoptosis in suspension cultures of tobacco, suggesting that hypersensitive response induced by PB90 is an apoptotic process.

机译：疫霉菌分泌的蛋白质激发子PB90是诱导烟草植物超敏反应和系统获得性抗性的有效激发子。在这里，我们观察了用台盼蓝染色法进行PB90处理的烟草BY-2悬浮培养细胞的细胞死亡。 cydoheximide是一种蛋白质合成抑制剂，可以抑制这种细胞死亡，这表明PB90诱导的细胞死亡是一个活跃的细胞死亡过程，需要新的蛋白质合成。 DAPI染色表明PB90诱导了快速的染色质浓缩，边缘化，凋亡小体形成和DMA阶梯化，进一步的TUNEL分析还观察到3'-OH末端的特异性断裂。上述所有共同的形态学特征表明，PB90诱导了烟草悬浮培养物中的细胞凋亡，这表明PB90诱导的超敏反应是一个凋亡过程。

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《Chinese science bulletin》 |2005年第5期|共5页
作者
JI Rui; ZHANG Zhengguang; WANG Yuanchao;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
Phytophthora elicitor PB90; apoptosis; DNA laddering; TUNEL;

机译：疫霉菌PB90;细胞凋亡;DNA标记;TUNEL;

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1. Phytophthora elicitor PB90 induced apoptosis in suspension cultures of tobacco [J] . JI Rui, ZHANG Zhengguang, WANG Yuanchao Chinese science bulletin . 2005,第5期

机译：疫霉PB90诱导烟草悬浮培养细胞凋亡。
2. The role of SA in the hypersensitive response and systemic acquired resistance induced by elicitor PB90 from Phytophthora boehmeriae. [J] . Zhang ZhengGuang, Wang YuanChao, Li Jun, Physiological and Molecular Plant Pathology . 2004,第1期

机译：SA在由疫霉疫霉的PB90诱导剂引起的过敏反应和全身获得性耐药中的作用。
3. Identification of the bacterial superoxide dismutase (SodM) as plant-inducible elicitor of an oxidative burst reaction in tobacco cell suspension cultures [J] . Watt SA, Tellstrom V, Patschkowski T, Journal of Biotechnology . 2006,第1期

机译：细菌超氧化物歧化酶（SodM）的鉴定为烟草细胞悬浮培养物中氧化猝灭反应的植物诱导型引发剂
4. A Tryptophan Pyrolysis Product, 3-Amino-1,4- dimethyl-5H-pyrido4,3-bindole(Trp-P-1) but Not Its Metabolite Induces Apoptosis in Primary Cultured Rat Hepatocytes: Induction of CYP 1A Interfaces Trp-P-1-Induced Apoptosis [C] . Bunsyo Shiotani, Hitoshi Ashida, Yuji Nonaka, Symposium on food factors in health promotion and disease prevention . 2003

机译：色氨酸热解产物，3-氨基-1,4-二甲基-5H-吡啶4,3-B吲哚（TRP-P-1）但不是其代谢物在原发性培养大鼠肝细胞中诱导细胞凋亡：CYP 1A界面的诱导TRP-P-1诱导的细胞凋亡
5. Auxin deprivation induces Golgi stack differentiation in suspension-cultured tobacco BY-2 cells [D] . Winicur, Zev Michael 1997

机译：生长素剥夺在悬浮培养的烟草BY-2细胞中诱导高尔基体堆栈分化
6. Induction of Proteinase Inhibitors in Tobacco Cell Suspension Culture by Elicitors of Phytophthora parasitica var. nicotianae [O] . Martina Rickauer, Joëlle Fournier, Marie-Thérèse Esquerré-Tugayé 1989

机译：烟草疫霉菌诱导子在烟草细胞悬浮培养中诱导蛋白酶抑制剂的研究。烟草科
7. Induction of Proteinase Inhibitors in Tobacco Cell Suspension Culture by Elicitors of Phytophthora parasitica var. nicotianae1 [O] . Rickauer, Martina, Fournier, Joëlle, Esquerré-Tugayé, Marie-Thérèse 1989

机译：烟草疫霉菌诱导剂在烟草细胞悬浮培养中诱导蛋白酶抑制剂的研究。烟草1

Phytophthora elicitor PB90 induced apoptosis in suspension cultures of tobacco

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