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首页> 外文期刊>Chinese science bulletin >Aluminium toxicity targets PIN2 in Arabidopsis root apices: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport
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Aluminium toxicity targets PIN2 in Arabidopsis root apices: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport

机译：铝毒性靶向拟南芥根尖中的PIN2：对PIN2内吞作用，囊泡回收和极性生长素运输的影响

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The most obvious symptom of Al toxicity is the inhibition of root growth. However, the mechanism of Al-inhibiting root growth remains to be elucidated. In this study, auxin transport and vesicle movement of an auxin-efflux carrier (PIN2) were investigated in Arabidopsis roots in response to Al stress. Results indicated that Al inhibited the apical transport of auxin in root tips of Arabidopsis significantly. The severe inhibition was localized in the cells of transition zone, where the concentration of auxin was only 34% that of the control. Brefeldin A (BFA), an inhibitor of vesicle transport, induced the dot-like structure of PIN2 vesicle significantly. Al decreased the size of dot-like structure of PIN2 vesicles. Results of real-time RT-PCR and Western-blotting analysis showed that Al increased the transcript level of PIN2 and the accumulation of PIN2 protein in horizontal direction of plasma membrane, but decreased its distribution in endosomes, suggesting that Al inhibited the transport of PIN2 vesicles from plasma membrane to endosomes. Results of cytoskeleton-depolymering drugs indicated that it was via the pathway of disruption of actin microfilaments that Al inhibited the transport of PIN2 vesicles. Exposed to Al stress, the cells of elongation zone had less Al uptake and less transport frequency of vesicles than cells of transition zone. Taken together, our results suggested that Al inhibited root growth mainly by modulating the transport of PIN2 vesicles between plasma membrane and endosomes, thus blocking auxin transport and root growth.
机译：Al毒性的最明显症状是根生长的抑制。然而，铝抑制根生长的机制仍有待阐明。在这项研究中，在拟南芥根中，针对铝胁迫，研究了植物生长素转运和植物生长素外排载体（PIN2）的囊泡运动。结果表明，铝显着抑制了拟南芥根尖中生长素的顶端转运。严重的抑制作用定位在过渡区的细胞中，其中生长素的浓度仅为对照的34％。布雷菲德菌素A（BFA），一种囊泡转运的抑制剂，可显着诱导PIN2囊泡的点状结构。 Al减小了PIN2囊泡的点状结构的尺寸。实时RT-PCR和Western印迹分析结果表明，Al增加了PIN2的转录水平和PIN2蛋白在质膜水平方向的积累，但减少了其在核内体中的分布，表明Al抑制了PIN2的转运从质膜到内体的囊泡细胞骨架解聚药物的结果表明，Al通过肌动蛋白微丝的破坏途径抑制了PIN2囊泡的运输。暴露于铝胁迫下，与过渡区相比，伸长区的细胞对铝的吸收较少，囊泡的运输频率也较低。两者合计，我们的研究结果表明铝主要通过调节PIN2囊泡在质膜和内体之间的运输来抑制根的生长，从而阻止生长素的运输和根的生长。

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