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首页> 外文期刊>Chinese science bulletin >Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae
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Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae

机译：重组家蚕幼虫口蹄疫病毒完整蛋白接种牛的免疫应答

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摘要

The intact open reading frame (ORF) of foot-and-mouth disease virus (FMDV) Asia I/XJ strain was amplified by RT-PCR and inserted into the transfer vector pVL1393 to generate plasmid pVL-ORF. Bm-N cells were transfected with pVL-ORF and linearized Sm-BacPAK6 DNA, and the recombinant silkworm baculovirus Sm-ORF containing the full ORF of FMDV was obtained. The results of indirect im-munofluorescence assay (IFA) showed that Bm-ORF could be expressed efficiently in Bm-N cell. After inoculating the early5th instar larvae of silkworm, the polyprotein of FMDV could be detected by sandwich ELISA and empty capsid-like particles could be observed under the electron microscope. Expression products from silkworm were used as the antigen to immunize the cattle.The specific antibody was induced in all vaccinated animals. The immunized cattle were challenged with the virulent FMDV Asia I/XJ strain, two of the four cattle were completely protected and clinical symptoms were alleviated and delayed in the others.The results suggest that this strategy might be used to develop the new subunit FMDV vaccine.
机译：通过RT-PCR扩增口蹄疫病毒（FMDV）亚洲I / XJ菌株的完整开放阅读框（ORF），并将其插入转移载体pVL1393中，以产生质粒pVL-ORF。用pVL-ORF和线性化的Sm-BacPAK6 DNA转染Bm-N细胞，得到含有FMDV完整ORF的重组家蚕杆状病毒Sm-ORF。间接免疫荧光分析（IFA）的结果表明，Bm-ORF可以在Bm-N细胞中有效表达。接种家蚕五龄初期幼虫后，可通过夹心ELISA检测FMDV的多蛋白，并在电子显微镜下观察到空衣壳样颗粒。用家蚕的表达产物作为抗原免疫牛。在所有接种疫苗的动物中诱导特异性抗体。用强毒的FMDV Asia I / XJ毒株对免疫牛进行了攻击，在四只牛中有两头受到了完全保护，另一只牛的临床症状得到缓解和延迟。 。

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