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首页> 外文期刊>Chinese science bulletin >Water-soluble chitosan nanoparticles as a novel carrier system for protein delivery
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Water-soluble chitosan nanoparticles as a novel carrier system for protein delivery

机译：水溶性壳聚糖纳米颗粒作为新型的蛋白质递送载体系统

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摘要

High MW chitosan (CS) solutions have already been proposed as vehicles for protein delivery. The aim of the present work is to investigate the potential utility of water-soluble chitosan (WSC) as vehicles to load and deliver proteins. WSC nanoparticles (WSC NP) with various formations were prepared based on ionic gelation of WSC with pentasodium tripolyphosphate (TPP) anions. Bovine serum albumin (BSA) was used as a model protein drug incorporated into the WSC nanoparticles. Blank and BSA-loaded WSC nanoparticles were examined and determined to have a spherical shape with diameters between 35—190 nm, and zeta potential between 35—42 mV. FTIR confirmed that the tripolyphos-phoric groups of TPP linked to the ammonium groups of WSC in the nanoparticles. Some factors affecting delivery properties of BSA have been investigated. Altering the concentration of BSA from 0.05 to 1 mg/mL enhanced the loading capacity of BSA but decreased loading efficiency simultaneously. Also, with the introduction of poly ethylene glycol (PEG), BSA release accelerated. Nanoparticle preparation from WSC with various deacetylation degrees (DDs) from 72.6% to 90% and MWs ranging from 3.5 to 15.8 kDa promoted loading efficiency and decreased the release rate. These results indicate that WSC nanoparticles are promising carriers for protein delivery.
机译：高分子量的壳聚糖（CS）解决方案已经被提出作为蛋白质输送的载体。本工作的目的是研究水溶性壳聚糖（WSC）作为装载和传递蛋白质的媒介的潜在用途。基于WSC与三聚磷酸五钠（TPP）阴离子的离子胶凝作用，制备了具有各种形成形式的WSC纳米颗粒（WSC NP）。牛血清白蛋白（BSA）被用作加入WSC纳米颗粒的模型蛋白药物。检查空白和BSA负载的WSC纳米颗粒，确定其直径为35-190 nm，ζ电位在35-42 mV之间的球形。 FTIR证实，TPP的三聚团与纳米颗粒中WSC的铵基相连。已经研究了影响BSA传递特性的一些因素。将BSA的浓度从0.05更改为1 mg / mL可以增强BSA的负载量，但同时会降低负载效率。此外，随着聚乙二醇（PEG）的引入，BSA的释放得以加速。由WSC制备的纳米颗粒具有不同的脱乙酰度（DDs）从72.6％至90％，分子量从3.5到15.8 kDa不等，可提高负载效率并降低释放速率。这些结果表明，WSC纳米颗粒是有希望的蛋白质递送载体。

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