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首页> 外文期刊>Chinese science bulletin >Transpositional behaviour of the Ds element in the Ac/Ds system in rice
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Transpositional behaviour of the Ds element in the Ac/Ds system in rice

机译：水稻Ac / Ds系统中Ds元素的转座行为

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Insertional mutagenesis based on maize Activator/Dissociator (AclDs) transposons is becoming a major approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA transformed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an AcIDs transposon system in rice. The successive investigation of Ds transposition from F_1 to F_5 generations indicated that the frequencies of germinal transposition increased over successive generations and reached 54.2% in F_3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking sequences of the Ds element were amplified. Analyses of 17 Ds-ftanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%.
机译：基于玉米激活子/解离子（AclDs）转座子的插入诱变正成为在水稻中产生饱和突变体集合的主要方法。在这项研究中，将Ds-T-DNA转化的纯合子与Ac-T-DNA转化的纯合子杂交，以在水稻中建立AcIDs转座子系统。从F_1到F_5代的Ds转座的连续研究表明，生发转座的频率在连续几代中增加，在F_3代中达到54.2％。 Ds换位模式显示，Ds换位引起T-DNA序列大约170 bp的缺失，另一个Ds换位带有272 bp的T-DNA序列。使用热不对称交错PCR（TAIL-PCR），扩增了Ds元件的一些侧翼序列。对17个Ds定位序列的分析表明，有5个Ds插入了基因区域。 D不仅可以转置到链接的站点，还可以转置到未链接的站点。 Ds的染色体间转座频率为33.3％。

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