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首页> 外文期刊>Chinese science bulletin >Morphological and phylogenetic analysis of a Gymnodinium-like species from the Chinese Coast
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Morphological and phylogenetic analysis of a Gymnodinium-like species from the Chinese Coast

机译：中国沿海裸Gym类物种的形态和系统发育分析

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A Gymnodinium-like species was studied with light microscopy (LM) and scanning electron microscopy (SEM). Also, the internal transcribed spacers (containing 5.8S rDNA) and large ribosomal subunit DNA (D1-D2) sequences were obtained by PCR amplification, and then sequenced to explore the relationships within our isolate, Gymnodinium and other Gymnodinium-like species, including Karenia, Gyrodinium, Karlodinium and Symbiodinium. The LM observation showed that the species was characterized by moving in a levorotatory direction, visible hypocone, epicone and transverse groove, all of which are typical for Gymnodinium. In addition, two flagella could be found under SEM. The phylogenetic analysis revealed that the isolate grouped with Symbiodium, rather than other relevant dinoflagellates. All results showed our isolate belongs to Symbiodium. The strain was isolated from a red tide water sample, denoting that Symbiodium may be causative species for algal bloom.
机译：用光学显微镜（LM）和扫描电子显微镜（SEM）研究了裸子草科动物。此外，通过PCR扩增获得了内部转录的间隔区（包含5.8S rDNA）和较大的核糖体亚基DNA（D1-D2）序列，然后进行了测序，以探索我们分离株，裸子草和其他裸子草样物种（包括卡伦尼亚）之间的关系。 ，Gyrodinium，Karlodinium和Symbiodinium。 LM观察表明，该物种的特征是沿左旋运动，可见的次圆锥，上圆锥和横沟，这都是裸子草属的典型特征。另外，在SEM下可以发现两个鞭毛。系统发育分析表明，该分离物与共生菌分组，而不是其他相关的鞭毛虫。所有结果表明我们的分离物属于共生菌属。该菌株是从赤潮水样品中分离出来的，表明共生菌可能是藻华的致病菌。

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